Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-03-27
1997-08-05
Campbell, Eggerton A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, C12Q 168, C12P 1934
Patent
active
056541430
ABSTRACT:
An RNA amplification method is particularly useful for diagnosing bacterial or viral infections or genetic disorders and for cell typing. The method includes the steps of denaturing a solution containing RNA, synthesizing a first cDNA strand from a suitable primer in the presence of reverse transcriptase, denaturing the heteroduplex formed, synthesizing a second cDNA strand from a second primer in the presence of DNA polymerase and then subjecting the cDNA formed to a sufficient number of amplification cycles. All the reactants and solvents are first placed in the same container to provide a single manipulation step that avoids the risk of contamination.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 5021335 (1991-06-01), Tecott et al.
patent: 5229297 (1993-07-01), Schnipelsky et al.
W. T. Tse et al., "Reverse Transcription and Direct Amplification of Cellular RNA Transcripts by Taq Polymerase", Gene, vol. 88, No. 2, Apr. 16, 1990, pp. 293-296.
E. Shimomaye et al., "Use of Avian Myeloblastosis Virus Reverse Transcriptase at High Temperature for Sequence Analysis of Highly Structured RNA", Gene Analysis Techniques, vol. 6, No. 2, Mar./Apr. 1989, pp. 25-28.
Goblet, C., "One-Step Amplification of Transcripts in Total RNA Using the Polymerase Chain Reaction", Nucleic Acids Research, vol. 17, No. 5, (1989) p. 2144.
Schwartz, S. et al., "Cloning and Functional Analysis of Muliply Spliced mRNA Species of Human Immunodeficiency Virus Type I", Journal of Virology, vol. 64, No. 6, Jun. 1990, pp. 2519-2529.
Nuovo, G., et al. "Detection of Human Papillomavirus DNA in Formalin-fixed Tissues by In Situ Hybridization After Amplification by Polymerase Chain Reaction", Am. J. Pathol., 139, 847-854 (1991).
Becker-Andre, M. et al., Absolute mRNA Quantification Using the Polymerase Chain Reaction (PCR). A Novel Approach by a PCR Aided Transcript Titration Assay (PATTY) Nucleic Acids Res., (17):9437-9447 (1989).
Chirgwin, J.M. et al., "Isolation of Biologically Active Ribonucleic Acid from Sources Enriched in Ribonuclease", Biochem., 18: 5294-5299 (1979).
Gilsin, V., et al. "Ribonucleic Acid Isolated by Cesium Chloride Centrifugation", Biochem., 13:2633-2638 (1973).
Myers, T.W., et al. "Reverse Transcription and DNA Amplification by a Thermus Thermophilus DNA Polymerase", Biochem., 30:7661-7666 (1991).
Robert-Guroff, M. et al., "Structure and Expression of tat-, rev-, and nef-Specific Transcripts of Human Immunodeficiency Virus Type 1 in Infected Lymphocytes and Macrophages", J. Virol., 64 (7):3391-3398 (1990).
Guatelli, J.C., et al., "Alternative Splice Acceptor Utilization during Human Immunodeficiency Virus Type 1 Infection of Cultured Cells", J. Virol., 64 (9):4093-4098 (1990).
Benko, D.M. et al., "A Novel Human Immunodeficiency Virus Type 1 Protein, tev, Shares Sequences with tat, env, and rev Proteins", J. Virol., 64:2505-2518 (1990).
Schwartz, S., et al., "Env and Vpu Proteins of Human Immunodeficiency Virus Type I Are Produced from Multiple Bicistronic mRNAs", J. Virol., 64 (11):5448-5456 (1990b).
Holland, S.M. et al., "Immunodefiency Virus Rev Protein Recognizes a Target Sequence in Rev-Responsive Element RNA within the Context of RNA Secondary Structure", J. Virol., 64 (12):5966-5975 (1990b).
Lawrence, J. et al. Human Retroviruses and Aids, (1990), edited by Myers, G., et al.
Mallet Fran.cedilla.ois
Mandrand Bernard
Oriol Guy
Bio Merieux
Campbell Eggerton A.
LandOfFree
RNA amplification method requiring only one manipulation step does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with RNA amplification method requiring only one manipulation step, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and RNA amplification method requiring only one manipulation step will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1074003