Chemistry: analytical and immunological testing – Process or composition for determination of physical state... – Corrosion resistance or power
Patent
1997-03-31
1998-10-06
Campbell, Eggerton A.
Chemistry: analytical and immunological testing
Process or composition for determination of physical state...
Corrosion resistance or power
435 912, C12Q 168, C12P 1934
Patent
active
058174656
ABSTRACT:
An RNA amplification method is particularly useful for diagnosing bacterial or viral infections or genetic disorders and for cell typing. The method includes the steps of denaturing a solution containing RNA, synthesizing a first cDNA strand from a suitable primer in the presence of reverse transcriptase, denaturing the heteroduplex formed, synthesizing a second cDNA strand from a second primer in the presence of DNA polymerase and then subjecting the cDNA formed to a sufficient number of amplification cycles. All the reactants and solvents are first placed in the same container to provide a single manipulation step that avoids the risk of contamination.
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Mallet Francois
Mandrand Bernard
Oriol Guy
Bio Merieux
Campbell Eggerton A.
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