Ripening inhibition in the tomato Green-ripe mutant...

Details Ripening inhibition in the tomato Green-ripe mutant... Ripening inhibition in the tomato Green-ripe mutant...

2011-05-24

2011-05-24

Baum, Stuart F. (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

C800S298000, C800S287000, C800S317400, C800S290000, C435S320100, C435S410000, C435S419000, C536S023600

Reexamination Certificate

active

07947867

ABSTRACT:
To achieve full development of the ripe phenotype, climacteric fruits, such as tomato, apple and banana, require synthesis, perception and signal transduction of the plant hormone ethylene. The non-ripening phenotype of the dominant Green-ripe (Gr) and Never-ripe 2 (Nr-2) mutants of tomato is the result of reduced ethylene responsiveness in fruit tissues. In addition a subset of ethylene responses associated with floral senescence, abscission and root elongation are also impacted in mutant plants but to a lesser extent. Using positional cloning we have identified an identical 334 by deletion in a gene of unknown biochemical function residing at the Gr/Nr-2 locus. Consistent with a dominant gain of function mutation, this deletion causes ectopic expression of GR/NR-2, which in turn leads to ripening inhibition. A CaMV35:GR transgene recreates the Gr/Nr-2 mutant phenotype but does not lead to a global reduction in ethylene responsiveness suggesting tissue-specific modulation of ethylene responses in tomato. GR/NR-2 encodes a novel evolutionary conserved membrane localized protein of unknown biochemical function that has not previously been associated with ethylene signaling. Because GR/NR-2 has no sequence homology with the previously described Nr (Never-ripe) ethylene receptor of tomato we now refer to this gene only as GR. Identification of GR expands the current repertoire of ethylene signaling components in plants and provides a tool for further elucidation of ethylene response mechanisms and for controlling ethylene signal specificity in crop plants.

REFERENCES:
Bowie et al (Science 247:1306-1310, 1990).
McConnell et al, Nature 411 (6838):709-713, 2001.
Benfey et al (1990, Science 250:959-966).
Benfey et al (1989, EMBO J, 8(8):2195-2202).
Barry et al (PNAS May 16, 2006, 103(20):7923-7928).

Affiliated with

Also associated with

Rating

Ripening inhibition in the tomato Green-ripe mutant... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Ripening inhibition in the tomato Green-ripe mutant..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Ripening inhibition in the tomato Green-ripe mutant... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2675692