Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-08-02
2005-08-02
McKelvey, Terry (Department: 1636)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C536S023200, C536S023100, C435S320100, C435S252300, C435S252330
Reexamination Certificate
active
06924106
ABSTRACT:
The present invention primarily relates to a DNA fragment which is obtainable from the gene cluster responsible for rifamycin biosynthesis within the genome ofAmycolatopsis mediterranei,and comprises at least one gene or a part of a gene which codes for a polypeptide which is directly or indirectly involved in the biosynthesis of rifamycin, and to a method for preparing said DNA fragment.The present invention furthermore relates to recombinant DNA molecules which comprise one of the DNA fragments according to the invention, and to the plasmids and vectors derived therefrom. Host organisms transformed with said plasmid or vector DNA are likewise embraced.
REFERENCES:
patent: 5763569 (1998-06-01), Brown et al.
patent: WO 87/03907 (1987-07-01), None
patent: WO 95/08548 (1995-03-01), None
Donadio et al., Science 252:675-679 (1991).
Aparicio et al., Gene, vol. 169, “Organization of the biosynthetic gene cluster for rapamycin inStreptomyces hygroscopicus: analysis of the enzymatic domains in the modular polyketide synthase,” pp. 9-16 (1996).
Bhat K.S., Gene, vol. 134, “Generation of a plasmid vector for deletion cloning by rapid multiple site-directed mutagenesis,” pp. 83-87 (1993).
Birnboim H.C., Methods in Enzymology, vol. 100, “A Rapid Alkaline Extraction Method for the Isolation of Plasmid DNA,” pp. 243-255 (1983).
Bonfield and Staden, Nucleic Acids Research, vol. 23 (8), “The application of numerical estimates of base calling accuracy to DNA sequencing projects,” pp. 1406-1410 (1995).
Chater K., Ciba Foundation Symposium 171, “Genetic regulation of secondary metabolic pathways inStreptomyces,” in Secondary Metabolites: Their Function and Evolution, John Wiley & Sons, pp. 144-162 (1992).
Donadio and Katz, Gene, vol. 111, “Organization of the enzymatic domains in the multifunctional polyketide synthase involved in erythromycin formation inSaccharopolyspora erythraea,” pp. 51-60 (1992).
Donadio et al., Science, vol. 252, “Modular Organization of Genes Required for Complex Polyketide Biosynthesis,” pp. 675-679 (1991).
Ghisalba et al., “The Rifamycins: Properties, Biosynthesis, and Fermentation,” inBiotechnology of Industrial Antibiotics, E.J. Vandamme (Ed.), pp. 281-327, Marcel Dekker, Inc. (1984).
Hopwood D.A., Current Opinion in Biotechnology, vol. 4, “Genetic engineering ofStreptomycesto create hybrid antibiotics,” pp. 531-537 (1993).
Hopwood et al.(ed.),Genetic Manipulation of Streptomyces—A Laboratory Manual, The John Innes Foundation, Norwich, pp. i-xii (1985)—preface and table of contents provided.
Hopwood et al., Ciba Foundation Symposium 171, “Genes for polyketide secondary metabolic pathways in microorganisms and plants,” inSecondary Metabolites: Their Function and Evolution, John Wiley & Sons, pp. 88-112 (1992).
Kieser and Melton, Gene, vol. 65, “Plasmid plJ699, a multi-copy positive-selection vector forStreptomyces,” pp. 83-91 (1988).
Lal et al., Critical Reviews in Microbiology, vol. 21(1), “Rifamycins: Strain Improvement Program,” pp. 19-30 (1995).
Lechevalier et al., Int. J. of Systematic Bacteriology, vol. 36 (1), Two New Genera of Nocardioform Actinomycetes:Amycolatagen. Nov. andAmycolatopsisgen nov., pp. 29-37 (1986).
Madon et al., J. of Bacteriology, vol. 173 (20), “Transformation System forAmycolatopsis(Nocardia)mediterranei:Direct Transformation of Mycelium with Plasmid DNA,” pp. 6325-6331 (1991).
Pospiech et al., Trends in Genetics, vol. 11 (6), “A versatile quick-prep of genomic DNA from Gram-positive bacteria,” pp. 217-128 (1995).
Schupp and Divers, FEMS Microbiology Letters, vol. 36, “Protoplast preparation and regeneration inNocardia mediterranei,” pp. 159-162 (1986).
Shupp et al., J. of Bacteriology, vol. 177 (13), “ASorangium cellulosum(Myxobacterium) Gene Cluster for the Biosynthesis of the Macrolide Antibiotic Soraphen A: Cloning, Characterization, and Homology to Polyketide Synthase Genes from Actinomycetes,” pp. 3673-3679 (1995).
Schwecke et al., Proc. Natl. Acad. Sci. USA, vol. 92, “The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin,” pp. 7839-7843 (1995).
Sensi et al., Farmaco Ed. Sci., vol. 14, “Rifomycin, A New Antibiotic—Preliminary Report,” pp. 146-147 (1959).
Smith et al., Methods Enzymol., vol. 151, “Strategies for Mapping and Cloning Macroregions of Mammalian Genomes,” pp. 461-489 (1987).
Thiemann et al., Arch. Mikrobiol., vol. 67, “A Proposal for the Transfer ofStreptomyces mediterraneiMargalith and Beretta 1960 to the GenusNocardiaasNocardia mediterranea(Margalith and Beretta) Comb. Nov.,” pp. 147-155 (1969).
Tsoi and Khosla, Chemistry & Biology, vol. 2, “Combinatorial biosynthesis of ‘unnatural’ natural products: the polyketide example,” pp. 355-362 (1995).
Wahl et al., Proc. Natl. Acad. Sci. USA, vol. 84, “Cosmid vectors for rapid genomic walking, restriction mapping, and gene transfer,” pp. 2160-2164 (1987).
Wehrli W., “Ansamycins—Chemistry, Biosynthesis, and Biological Activity,” inTopics in Current Chemistry, vol. 72, pp. 21-49 (1971).
Wehrli and Staehelin, Bacteriological Reviews, vol. 35 (3), “Actions of the Rifamycins,” pp. 290-309 (1971).
Stratmann et al., Microbiology (1999), 145, 3365-3375.
Schupp et al., FEMS Microbiology Letters 159 (1998) 201-207.
Yu, T. et al., (1999), PNAS USA 96, 9051-9056.
Engel Nathalie
Schupp Thomas
Toupet Christiane
McKelvey Terry
Novartis AG
Prince John T.
Vogel Nancy T.
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