Rice regulatory sequences for gene expression in defined...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C435S419000, C435S468000, C800S278000, C800S320300, C800S298000

Reexamination Certificate

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07375208

ABSTRACT:
The abundance of the 96 most abundant EST cluster sequences in a wheat lemma/palea cDNA library was investigated in a range of cDNA libraries made from various wheat tissues. 30 cDNA sequences showing highly enhanced abundance in lemma, palea and glume tissues over leaf, stem, embryo, endosperm and root tissue were selected for further analysis. These wheat EST cluster sequences were used to identify rice cDNA homologs. The abundance of the rice cDNA homologs was compared in rice leaf and panicle (includes lemma and palea) cDNA libraries. Rice cDNAs showing preferential expression in the panicle were then used to identify homologous rice genomic DNA clones, the putative promoter sequences have been identified and cloned.

REFERENCES:
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Cocciolone et al. Hierarchical patterns of transgene expression indicate involvement of developmental mechanisms in the regulation of the Maize P1-rr promoter. (2000) Genetics, vol. 156, pp. 839-846.
van Knippenberg et al. In vitro transcription of Tomato spotted wilt virus is independent of translation. (2004) J. of Gen. Virol., vol. 85, pp. 1335-1338.
Kay et al. Duplication of CaMV 35S Promoter Sequences Creates a Strong Enhancer for Plant Genes. (1987) Science, vol. 236, pp. 1299-1302.
Database EM—HTG, EBI Hinxton, GB AC/ID No. AP004068, Sasaki T et al.: “Oryza sativanipponbare (GA3) genomic DNA, chromosome 2, BAC”, XP002188680 (Aug. 17, 2001).

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