Rice-derived high expression polypeptide chain elongation...

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

Reexamination Certificate

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C435S320100, C435S419000, C800S287000, C536S024100

Reexamination Certificate

active

10473493

ABSTRACT:
DNA having promoter activity for strongly expressing an exogenous gene specifically in plant tissue (particularly, tissue having active cell prolifaration) is provided. The DNA is a rice OsEF1β1 gene promoter which has a sequence indicated by SEQ ID NO. 1, or a portion of said sequence whose promoter activity is equivalent to that of said sequence indicated by SEQ ID No. 1. An expression vector containing the promoter and an exogenous gene expressibly linked to the promoter is also provided. A plant cell transformed with the expression vector, a plant regenerated from the plant cell, a progeny of the plant, a propagator of the plant, and a seed obtained from the progeny are also provided. A method for introducing an exogenous gene into plants using an expression vector is also provided.

REFERENCES:
patent: 10-248570 (1998-09-01), None
Kim et al.,1994, Plant Molecular Biology 24: 105-117.
Oommenn et al.,1994, The Plant Cell 6:1789-1803.
Evans et al., 1992, Plant Mol. Biol. 20:1019-1028.
Peng et al., 1995, Plant Mol. Biol. 27:91-104.
Terui et al., A novel variant of translation elongation factor-1β: isolation and characterization of the rice gene encoding EF-1β2,Biochem. Biophys. Acta, 1442:369-372, (1998).
Matsumoto et al., “Cloning and characterization of the cDNA encoding rice elongation factor 1β”,FEBS Lett., 338(1):103-106, (1994).
McElroy et al., “Isolation of an Efficiant Actin Promoter for Use in Rice Transformation”,The Plant Cell, 2:163-171, (1990).
EMBL database accession No. AP003261,Oryza sativa(japonica cultivar group), 2001.
GenBank database accession No. AQ259567,Oryza sativa(japonica cultivar group), 1998.
GenBank database accession No. AQ330640,Oryza sativa(japonica cultivar group), 1999.

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