Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide contains a tissue – organ – or cell...
Reexamination Certificate
1998-06-25
2001-09-11
Benzion, Gary (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide contains a tissue, organ, or cell...
C435S069800, C435S320100, C435S468000, C536S023600, C536S024100, C800S298000, C800S320000, C800S320200
Reexamination Certificate
active
06288303
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates to novel rice &bgr;-glucanase enzymes and genes, and their uses in improved monocot plants.
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BACKGROUND OF THE INVENTION
The endo-1,3-&bgr;-glucanase gene family encodes isozymes that catalyze the hydrolysis of 1,3-&bgr;-D-glycosidic bonds in cell wall polymers of plants and fungi. The substrates for these glucanases include 1,3-&bgr;-glucans and 1,3;1,6-&bgr;-glucans. An N-terminal signal peptide directs all glucanase isozymes into the endoplasmic reticulum. Most glucanase isozymes are then secreted into the apoplast, but C-terminal peptides direct certain glucanase isozymes into the vacuole (Simmons, 1994).
This diversity of functions is matched by a multiplicity of 1,3-&bgr;-glucanase genes and isozymes. In the more exhaustively studied plant species, the number of genes or isozymes ranges from 5 to 14 (Simmons, 1994). The first &bgr;-glucanase gene characterized in rice was Gns1;1, which was predicted to encode a 1,3;1,4-&bgr;-glucanase based on homology to the EI gene of barley (Simmons, et al., 1992). Akiyama, et al. have recently characterized a glucanase isozyme from rice which has 1,3;1,4-&bgr;-glucanase activity and an acidic pI (Akiyama, et al., 1996a, 1996b); this isozyme may be encoded by the Gns1;1 gene. Akiyama, et al. have also recently characterized two 1,3-&bgr;-glucanase isozymes from rice (Akiyama, et al., 1996a, 1996b; Akiyama, et al., 1997).
Eight genes which encode novel rice &bgr;-glucanase isozymes Gns2-9 are discosed herein. The genes, the gene promoters, and nucleic acids encoding signal peptides, full-length proteins, and mature proteins, are useful in a variety of transgenic monocot plants, for example, in achieving increased plant resistance to fungal infection, improved growth characteristics, and high levels of expression of heterologous proteins in various tissues obtained from the plants.
SUMMARY OF THE INVENTION
The invention includes, in one aspect, an isolated DNA having a sequence of nucleotides which hybridizes under conditions of high stringency with a rice &bgr;-glucanase gene having one of the sequences: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, and SEQ ID NO:8, corresponding to rice &bgr;-glucanase genes 2-9 (Gns2-Gns9), respectively.
In one embodiment, the isolated DNA has a sequence with at least 80% nucleotide sequence identity to one of the rice Gns2-9 gene sequences identified as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO: 7, and SEQ ID NO: 8, or to a portion of one of these sequences as set out below. In other embodiments, the isolated DNA sequence has at least 90% sequence identity or at least 95% sequence identity to one of SEQ ID NO:1-8 or a portion thereof as described below.
The DNA may include the promoter region from one of the genes whose promoter regions are identified by SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO: 11, SEQ ID NO:12, SEQ ID NO: 13, SEQ ID NO:14, SEQ ID NO:15, or SEQ ID NO:16.
Alternatively, the DNA may include the signal-peptide coding sequence from one of the genes whose signal-peptide coding regions are identified by SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, or SEQ ID NO:31.
Alternatively, the DNA may include the coding sequence for one of the mature &bgr;-glucanase enzymes, whose coding regions are identified by SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:37, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, or SEQ ID NO:47.
In another aspect, the invention includes a chimeric gene for use in producing a transgenic monocot plant. The gene has a transcriptional regulatory region inducible during seed germination and effective to hybridize under conditions of high stringency with a rice &bgr;-glucanase gene promoter having one of the sequences SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO: 11, SEQ ID NO:12, SEQ ID NO: 13, SEQ ID NO:14, SEQ ID NO:15, or SEQ ID NO:16. Also included are a first DNA sequence heterologous to the regulatory region, and encoding a protein to be produced by the plant, and a second DNA sequence encoding a signal polypeptide. The second DNA sequence is operably linked to the transcriptional regulatory region and the first DNA sequence, and the signal polypeptide is in translation-frame with the protein and is effective to facilitate secretion of the protein across aleurone or scutellar epithelium layers into the endosperm of seeds obtained from the
Benzion Gary
Mehta Ashwin D.
The Regents of the University of California
Townsend and Townsend / and Crew LLP
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