Ribozymes with product ejection by strand displacement

Details Ribozymes with product ejection by strand displacement Ribozymes with product ejection by strand displacement

1994-04-22

1997-05-20

LeGuyader, John L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 4351721, 536 231, 536 232, 536 245, C12P 1934, C12Q 168, C07H 2100, C07H 2104

Patent

active

056311486

ABSTRACT:
Ribozymes designed to provide improved rates of catalytic turnover are described. The compounds of this invention comprise a catalytic region, at least one substrate binding region, and at least one displaceable antisense arm, whereby the rate of release of the endonuclease cleavage fragments is enhanced. A method to make such ribozymes is also described.

REFERENCES:
patent: 4818680 (1989-04-01), Collins et al.
patent: 4987071 (1991-01-01), Cech et al.
patent: 5037746 (1991-08-01), Cech et al.
patent: 5093246 (1992-03-01), Cech et al.
patent: 5116742 (1992-05-01), Cech et al.
patent: 5144019 (1992-09-01), Rossi et al.
patent: 5168053 (1992-12-01), Altman et al.
patent: 5180818 (1993-01-01), Cech et al.
patent: 5225337 (1993-07-01), Robertson et al.
patent: 5225347 (1993-07-01), Goldberg et al.
patent: 5254678 (1993-10-01), Haseloff et al.
patent: 5436330 (1995-07-01), Taira et al.
United States Biochemical Corp Catalog, p. 177.
Taira et al., "Construction of a novel artificial-ribozyme-releasing plasmid", Protien Eng. 3(8):733-737 (1990).
David P. Bartel and Jack W. Szostak: Isolation of New Ribozymes From a Large Pool of Random Sequences; Science, vol. 261; pp. 1411-1418; Sep. 10, 1993.
Malcolm J. Bennett and Julie V. Cullimore: Selective Cleavage of Closely-Related mRNAs by Synthetic Ribozymes; Nucleic Acids Research, vol. 20, No. 4, 831-837; 1992.
J.M. Buzayan, W. L. Gerlach and G. Bruening: Non-Enzymatic Cleavage and Ligation of RNAs Complementary to a Plant Virus Satellite RNA; Nature, vol. 323; pp. 349-353; Sep. 25, 1986.
J.M. Buzayan, A. Hampel & G. Bruening: Nucleotide Sequence and Newly Formed Phosphodiester Bond of Spontaneously Ligated Satellite Tobacco Ringspot Virus RNA; Nucleic Acids Research, vol. 14, No. 24, pp. 9729-9743, 1986.
T.A. Cha, J. Kolberg, B. Irvine, M. Stempien, E. Beall, M. Yano, Q. Choo, M. Houghton, G. Kuo, J.H. Han, and M.S. Urdea: Use of a Signature Nucleotide Sequence of Hepatitis C Virus for Detection of Viral RNA in Human Serum and Plasma; Journal of Clinical Microbiology, vol. 29, No. 11, pp. 2528-2534, Nov. 1991.
T.-A.Cha, E. Beall, B. Irvine, J. Kolberg, D. Chien, G. Kuo, and M.S. Urdea: At Least Five Related, But Distinct, Hepatitis C Viral Genotypes Exist; Proc. Natl. Acad. Sci. USA, vol. 89, pp. 7144-7148, Aug. 1992.
B.M. Chowrira and J.M. Burke: Extensive Phosphorothioate Substitution Yields Highly Active and Nuclease-Resistant Hairpin Ribozymes; Nucleic Acids Research, vol. 20, No. 11, 2835-2840, 1992.
M. Cotten, G. Schaffner, and M.L. Birnstiel: Ribozxyme, Antisense RNA, and Antisense DNA Inhibition of U7 Small Nuclear Ribonucleoprotein-Mediated Histone Pre-mRNA Processing In Vitro; Molecular and Cellular Biology, vol. 9, No. 10, pp. 4479-4487, Oct. 1989.
M.J. Fedor and O.C. Uhlenbeck: Kinetics of Intermolecular Cleavage by Hammerhead Ribozymes; Biochemistry, 31, 12042-12054, 1992.
John Goodchild and Vipin Kohli: Ribozymes That Cleave an RNA Sequence from Human Immunodeficiency Virus: The Effect of Flanking Sequence on Rate; Archives of Biochemistry and Biophysics, vol. 284, No. 2, pp. 386-391, Feb. 1, 1991.
John Goodchild: Enhancement of Ribozyme Catalytic Activity by a Contiguous Oligodeoxynucleotide (Facilitator) and by 2'-O-methylation; Nucleic Acids Research; vol. 20, No. 17, 4607-4612; 1992.
J.A. Grasby, P. Jonathan G. Butler and Michael Gait: The Synthesis of Oligoribonucleotides Containing O.sup.6 -methylguanosine: The Role of Conserved Guanosine Residues in Hammerhead Ribozyme Cleavage; Nucleic Acids Research, vol. 21, No. 19, pp. 4444-4450; Aug. 1993.
Sergei M. Gryaznov and David H. Lloyd: Modulation of Oligonucleotide Duplex and Triplex Stability Via Hydrophobic Interactions; Nucleic Acids Research, vol. 21, No. 25, 5909-5915, 1993.
Arnold Hampel and Richard Tritz: RNA Catalytic Properties of the Minimum (-)s TRSV Sequence; Biochemistry, vol. 28, No. 12, 4929-4933, 1989.
Jim Haseloff and Wayne L. Gerlach: Simple RNA Enzymes With New and Highly Specific Endoribonuclease Activities; Nature, vol. 334, pp. 585-591; Aug. 18, 1988.
Jim Haseloff and Wayne L. Gerlach: Sequences Required for Self-Catalyzed Cleavage of the Satellite RNA of Tobacco Ringspot Virus; Gene, 82, 43-52, 1989.
Olaff Heidenreich and Fritz Eckstein: Hammerhead Ribozyme-mediated Cleavage of the Long Terminal Repeat RNA of Human Immunodeficiency Virus type 1; The Journal of Biological Chemistry, vol. 267, No. 3, pp. 1904-1909, Jan. 25, 1992.
Olaf Heidenreich, Wolfgang Pieken and Fritz Eckstein: Chemically Modified RNA: Approaches and Applications; The FASEB Journal, vol. 7, pp. 90-96, Jan. 1993.
Philip Hendry, Maxine J. McCall, Fernando S. Santiago and Philip A. Jennings: Ribozyme with DNA in the Hybridizing Arms Displays Enhanced Cleavage Ability; Nucleic Acids Research, vol. 20, No. 21, pp. 5737-5741, 1992.
K.J. Hertel, A. Pardi, O.C. Uhlenbeck, M. Koizumi, E. Ohtsuka, S. Uesugi R. Cedergren, F. Eckstein, W.L. Gerlach, R. Hodgson and R.H. Symons: Numbering System for the Hammerhead; Nucleic Acids Research, vol. 20, No. 12, p. 3252, 1992.
Alex C. Jeffries and Robert W. Symons: A Catalytic 13-mer Ribozyme; Nucleic Acids Research, vol. 17, No. 4, pp. 1371-1377, 1989.
Makoto Koizumi, Shigenori Iwai and Eiko Ohtsuka: Cleavage of Specific Sites of RNA by Designed Ribozymes; FEBS Letters, vol. 239, No. 2, pp. 285-288; Nov. 1988.
Makoto Koizumi and Eiko Ohtsuka: Effects of Phosphorothioate and 2-Amino Groups in Hammerhead Ribozymes on Cleavage Rates and Mg.sup.2+ Binding; Biochemistry, vol. 30, No. 21, 51450-5150, 1991.
Makoto Koizumi, Yoji Hayase, Shigenori Iwai Hiroyuki Kamiya, Hideo Inoue and Eiko Ohtsuka: Design of RNA Enzymes Distinguishing a Single Base Mutation in RNA; Nucleic Acids Research, vol. 17, No. 17, pp. 7059-7071, 1989.
J.W. Lamb and R.T. Hay: Ribozymes That Cleave Potato Leafroll Virus RNA Within the Coat Protein and Polymerase Genes; Journal of General Virology, 71, pp. 2257-2264, 1990.
David M. Long and Olke C. Uhlenbeck: Self-Cleaving Catalytic RNA; The FASEB Journal, vol. 7, pp. 25-30, Jan. 1993.
Joshua O. Ojwang, Arnold Hampel, David J. Looney, Flossie Wong-Staal, and Jay Rappaport: Inhibition of Human Immunodeficiency Virus Type 1, Expressions By A Hairpin Ribozyme; Proc. Natl. Acad. Sci. USA, vol. 89, pp. 10801-10806, Nov. 1992.
David B. Olsen, Fritz, Benseler, Helle Aurup, Wolfgang A. Pieken and Fritz Eckstein: Study of Hammerhead Ribozyme Containing 2'-Modified Adenosine Residues; Biochemistry, vol. 30, pp. 9735-9741, 1991.
Jean-Pierre Perreault, Taifeng Wu, Benoit Cousineau, Kelvin K. Ogilvie and Robert Cetergren: Mixed Deoxyribo-and Ribo-Oligonucleotides with Catalytic Activity; Nature, vol. 344, pp. 565-567, Apr. 5, 1990.
Jean-Pierre Perreault, Damian Labuda, Nassim Usman, Jig-Hua Yang and Robert Cedergren: Relationship Between 2'-Hydroxyls and Magnesium Binding in the Hammerhead RNA Domain: A Model for Ribozyme Catalysis: Biochemistry vol. 30, No. 16, pp. 4020-4025, 1991.
Anne T. Perrotta and Michael D. Been: Cleavage of Oligoribonucleotides by a Ribozyme Derived from the Hepatitis Virus RNA Sequence, Biochemistry, vol. 31, No. 1, pp. 16-21, 1992.
Giovanni Paolella, Biran S. Sproat and Angus I. Lamond: Nuclease Resistant Ribozymes with High Catalytic Activity: The EMOB Journal, vol. 11, No. 5, pp. 1913-1919, 1992.
Wolfgang A. Pieken, David B. Olsen, Fritz Benseler, Heele Aurup, Fritz Eckstein: Kinetic Characterization of Ribonuclease-Resistant 2'-Modified Hammerhead Ribozymes; Science, vol. 253, pp. 314-317, Jul. 19, 1991.
Gerry A. Prody, John T. Bakos, Jamal M. Buzayan, Irving R. Schneider, George Bruening: Autolytic Processing of Dimeric Plant Virus Satellite RNA; Science, vol. 231, pp. 1577-1580; Mar. 28, 1986.
Duane E. Ruffner and Olke C. Uhlenbeck: Thiophosphate Interference Experiments Locate Phosphates Important for the Hammerhead RNA Self-Cleavage Reaction; Nucleic Acids Research, vol. 18, No. 20, pp. 6025-6029; 1990.
Susumu Shibahara, Sachiko Mukai, Hirokaza Morisawa, Kideki Nakashima, Susumu Kobayashi and Naoki Yamamoto: Inhibition of Human Immunodeficiency Virus (HIV-1) Replication by Synthetic Oligo-RNA Derivatives; Nuclei

Affiliated with

Also associated with

Rating

Ribozymes with product ejection by strand displacement does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Ribozymes with product ejection by strand displacement, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Ribozymes with product ejection by strand displacement will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1723163