Ribozymes with optimized hybridizing arms, stems, and loops, tRN

Details Ribozymes with optimized hybridizing arms, stems, and loops, tRN Ribozymes with optimized hybridizing arms, stems, and loops, tRN

1994-06-24

1999-12-07

Elliott, George C.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 9131, 4352523, 4353201, 435325, 435358, 435365, 435419, 536 245, C07H 2102, C12N 121, C12N 510, C12N 1570

Patent

active

059981938

ABSTRACT:
This invention is directed to improved catalytic compounds, hammerhead ribozymes, capable of hybridizing with a target RNA to be cleaved. These improved compounds have optimized stems (X)m * (X)m', loops (X)b and hybridizing arms. The invention is also directed to compositions for enhanced RNA cleavage which comprise a first synthetic non-naturally occurring oligonucleotide compound which comprises nucleotides whose sequence defines a conserved catalytic region and nucleotides whose sequence is capable of hybridizing with a predetermined target sequence and a second synthetic non-naturally occurring oligonucleotide which does not contain the predetermined target sequence and is complementary to at least a portion of the first oligonucleotide compound. The invention is also directed to synthetic non-naturally occurring oligonucleotide compounds embedded in a tRNA. The ribozymes and compositions of the present invention may be used in vitro or in vivo. They may be used as diagnostic or therapeutic agents.

REFERENCES:
patent: 5225337 (1993-07-01), Robertson et al.
patent: 5254678 (1993-10-01), Haseloff et al.
patent: 5298612 (1994-03-01), Jennings et al.
patent: 5334711 (1994-08-01), Sproat et al.

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