Ribozymes with broad tRNA aminoacylation activity

Details Ribozymes with broad tRNA aminoacylation activity Ribozymes with broad tRNA aminoacylation activity

2003-02-18

2009-11-24

Epps-Smith, Janet L (Department: 1633)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091310, C536S022100, C536S023100, C536S023200

Reexamination Certificate

active

07622248

ABSTRACT:
The present invention provides catalytic RNA molecules having cis or trans aminoacylation activity. The catalytic RNA molecules having cis aminoacylation activity comprise a catalytic domain and an aminoacylation domain. The catalytic RNA molecules having trans aminoacylation activity only have the catalytic domain. A method is provided for constructing and screening of these molecules. These molecules are suitable for aminoacylating with specific amino acids.

REFERENCES:
patent: 5270163 (1993-12-01), Gold et al.
patent: 5475096 (1995-12-01), Gold et al.
patent: 5871924 (1999-02-01), Yarus et al.
patent: 5990142 (1999-11-01), Carganico et al.
patent: 5998142 (1999-12-01), Gold et al.
patent: 6063566 (2000-05-01), Joyce
patent: 6280936 (2001-08-01), Burgin et al.
patent: WO 9936517 (1999-07-01), None
patent: WO 01/38582 (2001-05-01), None
patent: WO 0138582 (2001-05-01), None
Behrens et al. Tetrahedron, 2000, vol. 56, pp. 9443-9449.
Ma et al. Biochemistry, 1993, vol. 32, pp. 7939-7945.
Lee et al. Nature Structural Biology, vol. 7, No. 1, pp. 28-33, Jan. 2000.
Derwent Summary of WO9936517A2 in English.
Bartel, David P. et al., “Isolation Of New Ribozymes From A Large Pool Of Random Sequences,”Science, Sep. 10, 1993, pp. 1411-1418, vol. 261, No. 5127.
Beaudry, Amber A. et al., “Directed Evolution Of An RNA Enzyme,”Science, Jul. 31, 1992, pp. 635-641, vol. 257, No. 5070.
Brenner, Sydney et al., “Encoded Combinatorial Chemistry,”Proc. Natl. Acad. Sci. USA, Jun. 1992, pp. 5381-5383, vol. 89.
Fechter, Pierre et al., “Ribozyme Processed tRNA Transcripts With Unfriendly Internal Promoter For T7 RNA Polymerase: Production And Activity,”FEBS Letters, 1998, pp. 99-103, vol. 436.
Illangasekare, Mali et al., “Aminoacyl-RNA Synthesis Catalyzed By An RNA,”Science, Feb. 3, 1995, pp. 643-647, vol. 267, No. 5198.
Illangasekare, Mali et al., “Small-Molecule-Substrate Interactions With A Self-Aminoacylating Ribozyme,”J. Mol. Biol., 1997, pp. 631-639, vol. 268.
Illangasekare, Mali et al., “Specific, Rapid Synthesis Of Phe-RNA By RNA,”Proc. Natl. Acad. Sci. USA, May 1999, pp. 5470-5475, vol. 96.
Lee, Nick et al., “Ribozyme-Catalyzed tRNA Aminoacylation,”Nature Structural Biology, Jan. 2000, pp. 28-33, vol. 7, No. 1.
Lohse, Peter A. et al, “Ribozyme-Catalysed Amino-Acid Transfer Reactions,”Nature, May 30, 1996, pp. 442-444, vol. 381.
Lorsch, Jon R. et al., “In Vitro Evolution Of New Ribozymes With Polynucleotide Kinase Activity,”Nature, Sep. 1, 1994, pp. 31-36, vol. 371.
Pan, Tao et al., “In Vitro Selection Of RNAs That Undergo Autolytic Cleavage With Pb2+,”Biochemistry, Apr. 28, 1992, pp. 3887-3895, vol. 31, No. 16.
Piccirilli, Joseph A. et al., “Aminoacyl Esterase Activity Of The Tetrahymena Ribozyme,”Science, Jun. 5, 1992, pp. 1420-1424, vol. 256, No. 5062.
Prudent, James R. et al., “Expanding The Scope Of RNA Catalysis,”Science, Jun. 24, 1994, pp. 1924-1927, vol. 264, No. 5167.
Saito, Hirohide et al., “A Ribozyme Exclusively Aminoacylates The 3′-Hydroxyl Group Of The tRNA Terminal Adenosine,”J. Am. Chem. Soc., 2001, pp. 7178-7179, vol. 123.
Saito, Hirohide et al., “Concurrent Molecular Recognition Of The Amino Acid And tRNA By A Ribozyme,”RNA, 2001, pp. 1867-1878, vol. 7.
Suga, Hiroaki et al., “Structural And Kinetic Characterization Of An Acyl Transferase Ribozyme,”J. Am. Chem. Soc., 1998, pp. 1151-1156, vol. 120.
Bessho, et al.; A tRNA aminoacylation system for non-natural amino acids based on a programmable ribozyme; Nature Biotechnology, vol. 20, Jul. 2002; pp. 723-728; XP-002374062.
Saito, et al.; An in vitro evolved precursor tRNA with aminoacylation activity; The EMBO Journal, 2001, vol. 20, No. 7; pp. 1797-1806; XP-002430320.

Affiliated with

Also associated with

Rating

Ribozymes with broad tRNA aminoacylation activity does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Ribozymes with broad tRNA aminoacylation activity, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Ribozymes with broad tRNA aminoacylation activity will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4064549