Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-05-09
1996-12-31
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 245, 536 231, C12Q 168, C07H 2102, C07H 2104
Patent
active
055893325
ABSTRACT:
A system is described for the use of a ribozyme as a diagnostic tool for detecting the presence of a nucleic acid, protein, or other molecule, in which the formation of an active ribozyme and cleavage of an assayable marker is dependent on the presence or absence of the specific target molecule. The essential component is a ribozyme specifically but reversibly binding a selected target in combination with a labelled co-target, preferably immobilized on a support structure. When both the target and co-target are bound, the ribozyme cleaves the label from the co-target, which is then quantifiable. Since the ribozyme is reversibly bound by target and co-target, it can reassociate with additional co-target, cleaving more label, thereby amplifying the reaction signal. In one embodiment, the target is a nucleic acid hybridizing to complementary sequences that form part of the ribozyme; in a second embodiment, the target is a protein or other macromolecule which is bound by interactions with a portion of the ribozyme molecule in another embodiment, a thermostable ribozyme is used, so that improperly bound ribozyme is destabilized and inactive at elevated temperatures. A method for isolating regularable ribozymes is also disclosed. The regulatable ribozymes are useful in the method for detecting the presence of a specific macromolecule, or can be used in in vitro or in vivo methods for inactivation or activation of the cleavage of target RNA molecules.
REFERENCES:
patent: 4775619 (1988-10-01), Urdea
Noller, H. F., et al., "Unusual Resistance of peptidyl Transferase to Protein Extraction Procedures," Science, 256:1416-1419 (1992).
Altman, S., "Ribonuclease P: An Enzyme With a Catalytic RNA Subunit," in Adv. Enzymol., 62:1-36 (John Wiley & Sons, New York, 1989).
Beaudry, A., et al.,G., "Directed Evolution of an RNA Enzyme," Science, 257:635-641 (1992).
Bock, L. et al., "Selection of Single-Stranded DNA Molecules that Bind and Inhibit Human Thrombin," Nature, 355.564-566 (1992).
Branch, A. et al., "The Brotherhood of Circular RNA Pathogens: Viroids, Circular Satellites, and the Delta Agent," Semin. Virol., 1:143-152 (1990).
Brown, J. et al., "Characterization of RNAs from Thermophilic Bacteria," in Keystone RNA Processing Meeting, 55abs (Keystone, 1992).
Cech, T., "Ribozymes, Tools for Sequence-Specific Cleavage of RNA," Editorial Comments, 16:1-5 (U.S. Biochemicals Corp., Cleveland, 1989).
Ellington, A. D., et al., Nature, "In Vitro Selection of RNA Molecules that Bind Specific Ligands," 346:818-822 (1990).
Ellington, A. D., et al., "Selection In Vitro of Single-stranded DNA Molecules that Fold into Specific Ligand-binding Structures," Nature, 355:850-852 (1992).
Forster, A. C., et al., "External Guide Sequences for an RNA Enzyme," Science, 249:783-786 (1990).
George, S. T., et al., "Robozyme Accessibility of Potential Cleavage Sites in Hepatitis B Virus S Gene Messenger RNA," Abstracts: Molecular Biology of Hepatitis B Viruses, 131 (La Julia, CA, 1992).
Haseloff, J., et al., "Simple RNA Enzymes with New and Highly Specific Endoribunuclease Activities," Nature, 334:585-591 (1988).
Herschlag, D., et al., "DNA Cleavage Catalysed by the Ribozyme from Tetrahymena," Nature, 344:405-409 (1990).
Kuo, M. Y. -P.,et al., "Characterization of Self-Cleaving RNA Sequences on the Genome and Antigenome of Human Hepatitis Delta Virus," Journal of Virology, 62(12):4439-4444 (1988).
Perotta, A. T., et al., "The Self-cleaving Domain from the Genomic RNA of Hepatitis Delta Virus: Sequence Requirements and the Effects of Denaturant," Nucleic Acids Res., 18(23):6821-6827 (1990).
Perotta, A. T., et al., "A Pseudoknot-like Structure Required for Efficient Self-cleavage of hepatitis Delta Virus RNA," Nature, 350:434-436 (1991).
Perotta, A. T., et al., "Cleavage of Oligoribunucleotides by a Ribozyme Derived from the Hepatitis .delta. Virus RNA Sequence," Biochemistry, 31:16-21 (1992).
Piccirilli, J. A., et al., "Aminoacyl Esterase Activity of the Tetrahymena Ribozyme," Science, 256:1420-1424 (1992).
Robertson, D., et al., "Selection In Vitro of an RNA Enzyme that Specifically Cleaves Single-stranded DNA," Nature, 344:467-469 (1990).
Rosenstein, S.P., et al., "Self-cleavage of hepatitis Delta Virus Genomic Strand RNA is Enchanced under Partially Denaturing Conditions," Biochemistry, 29:8011-8016 (1990).
Sharmeen et al., "Antigenomic RNA of Human Hepatitis Delta Virus Can Underto Self-cleavage," J. Virol., 62:2674-2679 (1988).
Shih et al., "Efficient Trans-cleavage and Re-targeting of hepatitis Delta Virus Ribozymes," Abstracts: Molecular Biology of Hepatitis B Viruses, 130 (La Jolla, CA, 1992).
Smith, J. B., et al., "Antigenomic Hepatitis Delta Virus Ribozymes Self-cleave in 18 M Formamide," Nucleic Acids Research, 19(6):1285-1289 (1991).
Symons, R. H., et al., "Ribozymes," Critical Reviews in Plant Sciences, 10(3):189-234 (CRC Press, Inc. Boca Raton, Fla. 1991).
Szostak, J. W., "In Vitro Genetics," Trends Biochem. Sci., 17:89-93 (1992).
Uhlenback, O. C., "A Small Catalytic Oligoribonucleotide," Nature, 328:596-600 (1987).
Weizsacker, F. von., et al., "Cleavage of hepatitis B Virus RNA by Three Ribozymes Transcribed from a Single DNA Template," Biochem. Biophys. Res. Comm., 189(2):743-748 (1992).
Wu, H-N, et al., "Reversible Cleavage and Ligation of Hepatitis Delta Virus RNA," Science, 243:652-655 (1989).
Wu, H-N, et al., "RNA Conformational Requirements of Self-cleavage of Hepatitis Delta Virus RNA," Mol. Cell. Biol., 10(10):5575-5579 (1990).
Wu, H-N, et al., "Human hepatitis .delta. Virus RNA Subfragments Contain an Autocleavage Activity," Proc. Natl. Acad. Sci. USA, 86:1831-1835 (1989).
Yuan, Y., et al., "Targeted Cleavage of mRNA by Human RNase P." Proc. Natl. Acad. Sci. USA, 89:8006-8010 (1992).
Zaug, A. J., et al., "The Intervening Sequence of RNA of Tetrahymena is an Enzyme," Science, 231:470-475 (1986).
Forester et al. Cell 49:211-220 (1987).
Bockman Jeffrey M.
George Shaji T.
Shih Andy
Innovir Laboratories, Inc.
Jones W. Gary
Pabst Patrea L.
Whisenant Ethan
LandOfFree
Ribozyme amplified diagnostics does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Ribozyme amplified diagnostics, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Ribozyme amplified diagnostics will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1141209