Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1998-05-05
2000-04-25
Brusca, John S.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 231, 536 2433, C12Q 168, C12N 2511
Patent
active
060542780
ABSTRACT:
The invention relates the discovery that the polymorphisms in 16S rRNA genes may be used to identify unknown bacterial isolates at the level of species, subspecies and strain identification. The invention also relates to the discovery that the sequences of individual are required. The invention involves the generation of composite polynucleotide sequence of the 16S rRNA genes of a microorganism of interest. The invention is to provide methods for identifying microorganisms. The methods comprise the steps of generating a composite 16S rRNA region sequence from a microorganism of interest. The composite 16S rRNA region sequence reveals polymorphisms within the 16S ribosomal RNA gene of the microorganism. The composite 16S rRNA region sequence may then be compared with a list of previously obtained composite 16S rRNA region sequences from reference microorganisms so as to determine the species, subspecies, or strain of the microorganism of interest. Another aspect of the invention is to provide computer based methods of identifying a microorganism.
REFERENCES:
Innis et al. DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA. Proc. Natl. Acad. Sci. USA vol. 85, pp. 9436-9440, 1988.
Clayton et al. Intraspecific variation in small-subunit rRNA sequences in GenBank: Why single sequences may not adequately represent prokaryotic taxa. Int. J. System. Bact. pp. 595-599, 1995.
Relman et al. The agent of bacillary angiomatosis. New Engl. J. Med. vol. 323 pp. 1573-1580, 1990.
Dodge Deborah E.
Smith Douglas H.
Bortner Scott R.
Brusca John S.
The Perkin-Elmer Corporation
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