Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-09-12
1997-12-16
Jones, W. Gary
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 435 9131, 435 9151, 435 9153, 514 44, 536 245, C12P 1934, C12Q 168, C07H 2102, C07H 2104
Patent
active
056984219
ABSTRACT:
The present invention provides new methods, employing a nucleotide integrase, for cleaving double-stranded and single stranded DNA substrates at specific sites and for attaching nucleic acid molecules to the cleaved DNA substrates. One method uses a nucleotide integrase to cleave one strand of a double-stranded DNA and to concomitantly attach a nucleic acid molecule to the cleaved strand. Another method uses a nucleotide integrase to cleave both strands of a double-stranded DNA substrate and to attach a nucleic acid molecule to one strand of the DNA substrate. Another method uses a nucleotide integrase to cleave both strands of a double-stranded DNA substrate and to attach an RNA molecule to one strand of the substrate and for attaching a cDNA to the other strand of the substrate. Another method cleaves single stranded DNA with the concomitant insertion of a nucleic acid molecule at the cleavage point. The nucleotide integrase comprises an RNP particle which comprises a group II intron RNA bound to a group II intron encoded protein. The present invention also relates to purified and reconstituted RNP particles and reconstituted RNP that cleave DNA substrates.
REFERENCES:
patent: 5498531 (1996-03-01), Jarrell
"Group I and group II introns" by Saldanha, et al. The FASEB Journal, vol. 7, Jan. 1993, pp. 15-24.
"Group II Intron Mobility Occurs to Target DNA-Primed Reverse Transcription" by Zimmerly, et al. Cell, vol. 82, Aug. 25, 1995, pp. 545-554.
"An Expanding Universe of Introns" by Belfort Science, vol. 262, Nov. 12, 1993, pp. 1009-1010.
"Integration of Group II Intron bl1 into a Foreign RNA by Reversal of the Self-Splicing Reaction in Vitro" by Morl and Schmelzer Cell, vol. 60, Feb. 23, 1990, pp. 629-636.
"A Group II Intron RNA is a Catalytic Component of a DNA Endonuclease Involved in Intron Mobility" by Zimmerly, et al., Cell, vol. 83, Nov. 17, 1995, pp. 1-10.
"RNA enzymes (ribozymes) as antiviral therapeutic agents" by Rossi and Sarver Tibtech vol. 8, Jul. 1990, pp. 179-183.
"Mobile Group II Introns of Yeast Mitochondrial DNA Are Novel Site-Specific Retroelements" by Moran, et al., Molecular and Cellular Biology, vol. 15, No. 5, May 1995, pp. 2828-2838.
"Evolutionary relationships among group II intron-encoded proteins and identification of a conserved domain that may be related to maturase function" by Mohr, et al., Nucleic Acids Research vol. 21, No. 22, 1993, pp. 4991-4997.
"Reverse Transcriptase Activity Associated with Maturase-Encoding Group II Introns in Yeast Mitochondria" by Kennell, et al., Cell, vol. 73, Apr. 9, 1993, pp. 133-146.
"Introns as Mobile Genetic Elements" by Lambowitz et al., Annual Reviews Biochem., vol. 62, pp. 587-622, 1993.
Guo Huatao
Lambowitz Alan M.
Yang Jian
Zimmerly Steven
Jones W. Gary
The Ohio State Research Foundation
Tran Paul B.
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