Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-04-29
1999-07-06
Ketter, James
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
4351723, 4352351, 536 231, 536 241, C12Q 168, C12N 1563, C12N 700, C07H 2104
Patent
active
059196254
ABSTRACT:
The present invention is directed to the process of creating a recombinant nucleic acid standard which is resistant to ribonuclease digestion and is non-infectious. A single strand of recombinant nucleic acid is encapsidated by bacteriophage proteins. The recombinant nucleic acid is a hybrid sequence encoding bacteriophage proteins and a specific non-bacteriophage sequence. A non-bacteriophage RNA sequence can be used as an RNA standard to help quantify the number of RNA molecules in an unknown sample. The recombinant RNA in its packaged form is highly resistant to ribonucleases, insuring that the RNA standard is not compromised by inadvertent ribonuclease contamination. These ARMORED RNA.TM. standards are ideal as RNA standards for the quantification of RNA viruses such as HIV and HCV from human body fluids such as blood and cerebrospinal fluid.
REFERENCES:
patent: 5091309 (1992-02-01), Schlesinger et al.
patent: 5443969 (1995-08-01), Wilson et al.
patent: 5500360 (1996-03-01), Ahlquist et al.
patent: 5578473 (1996-11-01), Palese et al.
patent: 5589367 (1996-12-01), Donson et al.
patent: 5602242 (1997-02-01), Ahlquist et al.
patent: 5618699 (1997-04-01), Hammamoto et al.
patent: 5622705 (1997-04-01), Morrow
patent: 5627060 (1997-05-01), Ahlquist et al.
patent: 5716821 (1998-02-01), Wertz et al.
patent: 5739026 (1998-04-01), Garoff et al.
Argetsinger and Gussin, "Intact Ribonucleic Acid from Defective Particles of Bacteriophage R17," J Mol Biol, 21:421-434, 1966.
Aslanzadeh et al., "Evaluation of PCR and Nested PCR for Laboratory Diagnosis of Hepatitis C Virus Infection," Mol Cell Probes, 10:173-178, 1996.
Barlow et al., "Analysis and Genotyping of PCR Products of the Amplicor HIV-1 Kit," J Virological Methods, 52:65-74, 1995.
Collins et al., "Preparation and Characterization of RNA Standards for Use in Quantitative Branched DNA Hybridization Assays," Analytical Biochemistry, 226:120-129, 1995.
Golmohammadi et al., "The Refined Structure of Bacteriophage MS2 at 2.multidot.8 .ANG. Resolution," J Mol Biol, 234:620-639, 1993.
Heisenberg, "Formation of Defective Bacteriophage Particles by fr Amber Mutants," J. Mol. Biol., 17:136-144, 1966.
Hughes and Andrew, "Creation of Deletion, Insertion and Substitution Mutations Using a Single Pair of Primers and PCR," Biotechniques, 20(2):188-196, 1996.
LeCuyer et al., "Mutants of the Bacteriophage MS2 Coat Protein that Alter its Cooperative Binding," Biochem, 34:10600-10606, 1995.
Lim and Peabody, "Mutations that Increase the Affinity of a Translational Repressor for RNA," Nuc Acids Res, 22(18):3748-3752, 1994.
Lim et al., "Altering the RNA Binding Specificity of a Translational Repressor," J Biol Chem, 269(12):9006-9010, Mar. 1994.
Lu and Andrieu, "Use of the Human Immunodeficiency Virus Virion as a Universal Standard for Viral RNA Quantitation by Reverse Transcription-Linked Polymerase Chain Reaction," J Infect Diseases, 167:1499-1500, 1993.
Mastico et al., "Multiple Presentation of Foreign Peptides on the Surface of an RNA-Free Sphereical Bacteriophage Capsid," J General Virology, 74:541-548, 1993.
Mellors et al., "Prognosis in HIV-1 Infection Predicted by the Quantity of Virus in Plasma," Science, 272:1167-1170, May 1996.
Mulder et al., "Rapid and Simple PCR Assay for Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma: Application to Acute Retroviral Infection," J Clin Microbiol, 32(2):292-300, Feb. 1994.
Pachl et al., "Rapid and Precise Quantification of HIV-1 RNA in Plasma Using a Branched DNA Signal Amplification Assay," J Acquired Immune Deficiency Syndromes and Human Retrovirology, 8:446-454, 1995.
Peabody and Ely, "Control of Translational Repression by Protein-Protein Interactions," Nuc Acids Res, 20(7):1649-1655, 1992.
Peabody and Lim, "Complementation of RNA Binding Site Mutations in MS2 Coat Protein Heterodimers," Nuc Acids Res, 24(12):2352-2359, 1996.
Peabody, "Translation Repression by Bacteriophage MS2 Coat Protein Expressed from a Plasmid," J Biol Chem, 265(10):5684-5689, Apr. 1990.
Piatak et al., "Quantitative Competitive Polymerase Chain Reaction for Accurate Quantitation of HIV DNA and RNA Species," Biotechniques, 14(1):70-79, 1993.
Pickett and Peabody, "Encapsidation of Heterologous RNAs by Bacteriophage MS2 Coat Protein," Nuc Acids Res, 21(19):4621-4626, 1993.
Pushko et al., "Analysis of RNA Phage fr Coat Protein Assembly by Insertion, Deletion and Substitution Mutagenesis," Protein Engineering, 6(8):883-891, 1993.
Qiao et al., "Interference with Bacteriophage .phi.6 Genomic RNA Packaging by Hairpin Structures," J Virology, 69(9):5502-5505, Sep. 1995.
Schneeberger and Zeillinger, "PCR-Mediated Synthesis of Exogenous Competitors for Quantitative RT-PCR," Biotechniques, 20(3):360-361, 1996.
Shaklee et al., "Infectious Positive- and Negative-Strand Transcript RNAs from Bacteriophage Q.beta. cDNA Clone," Virology, 163:209-213, 1988.
Shaklee, "Negative-Strand RNA Replication by Q.beta. and MS2 Positive-Strand RNA Phage Replicases," Virology, 178:340-343, 1990.
Shiba and Saigo, "Packaging of tRNA and 6 S Stable RNA into Bacteriophage MS2 Particles Produced in Escherichia coli Treated with 5-Fluorouracil," Virology, 119:209-213, 1982.
Shiba and Suzuki, "Localization of a Protein in the RNA-A Protein Complex of RNA Phage MS2," Biochimica et Biophysica Acta, 654:249-255, 1981.
Stockley et al., "Molecular Mechanism of RNA Phage Morphogenesis," Int. J. Biochem., 26(10/11)1249-1260, 1994.
Stockley et al., "Probing Sequence-Specific RNA Recognition by the Bacteriophage MS2 Coat Protein," Nuc Acids Res, 23(13):2512-2518, 1995.
Valegard et al., "Crystal Structure of an RNA Bacteriophage Coat Protein-Operator Complex," Nature, 371:623-626, Oct. 1994.
Valegard et al., "The Three-dimensional Structure of the Bacterial Virus MS2," Nature, 345:36-41, May 1990.
van Gemen, "A One-Tube Quantitative HIV-1 RNA NASBA Nucleic Acid Amplification Assay Using Electrochemiluminescent (ECL) Labelled Probes," J Virological Methods, 49:157-168, 1994.
Witherell et al., "Specific Interaction Between RNA Phage Coat Proteins and RNA," Proc Nuc Acid Res Molec Biol, 40:185-220, 1991.
Witherell et al., "Cooperative Binding of R17 Coat Protein to RNA," Biochem, 29:11051-11057, 1990.
Young et al., "Detection of Hepatitis C Virus RNA by a Combined Reverse Transcription-Polymerase Chain Reaction Essay," J Clinical Microbiol, 31(4):882-886, Apr. 1993.
Dolja et al., "Phylogeny of Capsid Proteins of Rod-Shaped and Filamentous RNA Plant Viruses: Two Families with Distinct Patterns of Sequence and Probably Structure Conservation," Virology, 184:79-86, 1991.
Duda et al., "Expression of Plasmid-Encoded Structural Proteins Permits Engineering of Bacteriophage T4 Assembly," Virology, 179:728-737, 1990.
Durham, "Structures and Roles of the Polymorphic Forms of Tobacco Mosaic Virus Protein," J. Mol. Biol., 67:289-305, 1972.
Durham, "The Cause of Irreversible Polymerisation of Tobacco Mosaic Virus Protein," FEBS Letters, 25(1):147-152, 1972.
Fritsch et al., "Specificity of TMV RNA Encapsidation: in vitro Coating of Heterologous RNA by TMV Protein," Virology, 56:33-45, 1973.
Gal-On et al., "Nucleotide Sequence of the Zucchini Yellow Mosaic Virus Capsid-Encoding Gene and its Expression in Escherichia coli," Gene 87:273-277, 1990.
Gallie et al., "In Vivo Uncoating and Efficient Expression of Foreign mRNAs Packaged in TMV-Like Particles," Science, 236:1122-1124, 1987.
Gallie et al., "The Effect of Multiple Dispersed Copies of the Origin-of-Assembly Sequence From TMV RNA on the Morphology of Pseudovirus Particles Assembled In Vitro," Virology, 158:473-476, 1987.
Gibbs, "Tobamovirus Group," C.M.I./A.A.B. Descriptions of Plant Viruses, No. 184, Commonwealth Bureaux and the Association of Applied Biologists, Sep. 1977.
Goelet et al., "Nucleotide Sequence of Tobacco Mosaic Virus RNA," Proc. Natl. Acad. Sci. USA, 79:5818-5922, 1982.
Goulden et al., "Structure of Tobraviral Particles: A Model Suggested From Sequence Conservation in Tobraviral and Tobamoviral Coat Proteins," J. Mol. Biol., 227:1-8, 1992.
Guilley et al., "Observations Concerning the Sequence of Two Addit
DuBois Dwight B.
Pasloske Brittan L.
Winkler Matthew M.
Ambion Inc.
Cenetron Diagnostics LLC
Ketter James
Sandals William
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