Riboflavin synthesis in yeast

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing heterocyclic carbon compound having only o – n – s,...

Reexamination Certificate

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Reexamination Certificate

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06929933

ABSTRACT:
The invention describes the riboflavin biosynthesis gene and gene products from yeast. Vectors and recombinant preparation processes for riboflavin are furthermore described. Specifically, the invention relates to the genes for riboflavin biosynthesis in yeast, the proteins encoded therewith and genetic engineering process for the preparation of riboflavin using these genes and gene products. Accordingly, six genes (rib genes), which encode from enzymes of riboflavin biosynthesis starting from GTP, were found in the yeastSaccharomyces cerevisiaeand isolated.

REFERENCES:
patent: 0 405 370 (1991-01-01), None
Revuelta et al. (1990) Biosynthesis of Vitamin B2 in Yeast. In Organizing Committee of Biotec-90 (Eds). From Genes to Bioproducts, DM PPU, Murcia, Spain, pp. 117-122.
Oltmanns et al. Biochemical and Genetic Classification of Riboflavine Deficient Mutants of Saccharomyces cerevisiae. Molec. Gen. Genetics (1969) 105: 306-313.

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