Ri T-DNA promoters

Details Ri T-DNA promoters Ri T-DNA promoters

1995-06-02

1996-08-06

Wax, Robert A.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

4352522, 800205, C07K 100, C12N 120, A01H 104

Patent

active

055435017

ABSTRACT:
The sequence of the T.sub.L -DNA of Ri plasmids found in Agrobacterium rhizogenes strains HRI and A4 is disclosed. Sixteen open reading frames bounded by eukaryotic promoters, ribosome binding sites, and polyadenylation sites were found, five of which were observed to be transcripted in a developmentally and phenotypically regulated manner. The use of promoters and polyadenylation sites from pRi T.sub.L -DNA to control expression of heterologous foreign structural genes is taught, using as examples the structural genes for Phaseolus vulgaris storage protein (phaseolin), P. vulgaris lectin, a sweet protein (thaumatin), and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.

REFERENCES:
patent: 4459355 (1984-07-01), Cello et al.
patent: 4771002 (1988-09-01), Gelvin
patent: 4956282 (1990-09-01), Goodman et al.
Hoekema et al. (1984) J. Bacteriology 158(1): 383-385.
Lahners et al. (1984) Plasmid 11(2): 130-140.
Hooykaas et al. (1984) Plasmid 11(3): 195-205.
Durand-Tardif et al. (1985) J. Mol. Biol. 186(3): 557-564.
Slightom et al. (1986) J. Biol. Chem. 26(1): 108-121.
Furner et al. (1986) Nature 319: 422-427.
Aoki et al. (1994) Mol. Gen. Genet. 243(6): 706-710.

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