RI T-DNA Promoters

Details RI T-DNA Promoters RI T-DNA Promoters

1994-11-08

1995-11-14

Wax, Robert A.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

4351723, 4352404, 4352522, 536 231, 800205, 935 6, 935 35, 935 36, C07H 1700

Patent

active

054667925

ABSTRACT:
The sequence of the T.sub.L -DNA of Ri plasmids found in Agrobacterium rhizogenes strains HRI and A4 is disclosed. Sixteen open reading frames bounded by eukaryotic promoters, ribosome binding sites, and polyadenylation sites were found, five of which were observed to be transcripted in a developmentally and phenotypically regulated manner. The use of promoters and polyadenylation sites from pRi T.sub.L -DNA to control expression of heterologous foreign structural genes is taught, using as examples the structural genes for Phaseolus vulgaris storage protein (phaseolin), P. vulgaris lectin, a sweet protein (thaumatin), and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.

REFERENCES:
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Jouanin, L. (1984) Plasmid 12:91-102.
Pomponi, M. et al., (1983) Plasmid 10:119-129.
Hernalsteens, J.-P. et al. (1984) EMBO J. 3:3039-3041.
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van Slogteren, G. M. S. (1984) Nature 311:763-764.
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Green (1982) Plant Tissue Culture, Proc. 5th Intl. Congress, pp. 107-108.
Hookyoas-Van, Slogtern (1984) Nature 311:763-764.
Huffman et al. (1984) J. Bacteriol. 157:269-276.
Jouanin (1984) Plasmid 12:91-102.
Pomponi et al. (1983) Plasmid 10:119-129.

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