Ri T-DNA promoters

Details Ri T-DNA promoters Ri T-DNA promoters

1995-06-02

1998-10-20

Fox, David T.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

800255, 536 237, 536 241, 4351723, 4352522, 435419, A01H 500, C12N 504, C12N 1531, C12N 1584

Patent

active

058248668

ABSTRACT:
The sequence of the T.sub.L -DNA of Ri plasmids found in Agrobacterium rhizogenes strains HRI and A4 is disclosed. Sixteen open reading frames bounded by eukaryotic promoters, ribosome binding sites, and polyadenylation sites were found, five of which were observed to be transcripted in a developmentally and phenotypically regulated manner. The use of promoters and polyadenylation sites from pRi T.sub.L -DNA to control expression of heterologous foreign structural genes is taught, using as examples the structural genes for Phaseolus vulgaris storage protein (phaseolin), P. vulgaris lectin, a sweet protein (thaumatin), and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.

REFERENCES:
patent: 5182200 (1993-01-01), Slightom et al.
patent: 5366887 (1994-11-01), Slightom et al.
Haffman et al. 1984, J. Bacteriol. 157: 269-276.
Barker et al. 1983, Plant Mol. Biol. 2:335-350.
Herrera-Estrella et al. 1983, Nature 303:209-213.
Zambryski et al. 1983, EMBO J 2(12):2143-2150.
Goodman et al. 1987. Science 236:48-54.

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