Rho target protein Rho-kinase

Drug – bio-affecting and body treating compositions – Enzyme or coenzyme containing – Transferases

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435 4, 435194, C12N 912, C12Q 100, A61K 3851

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active

059068191

ABSTRACT:
The object of the present invention is to provide a target protein for the activated Rho protein. The present invention is a protein having activated Rho protein binding activity and protein kinase activity or derivatives thereof. The molecular weight of the protein derived from bovine is about 164 kDa as measured by SDS-PAGE. The protein kinase activity of this protein is enhanced when it binds to the activated Rho protein.

REFERENCES:
Amano et al., "Identification of A Putative Target For Rho As The Serine-Threonine Kinase Protein Kinase N", Science, vol. 271:648-650, (1996).
Ando et al., "Post-Translation Processing Of rac p21s Is Important Both For Their Interaction With The GDP/GTP Exchange Proteins And For Their Activation of NADPH Oxidasde," The Journal Of Biological Chemistry, vol. 267(36):25709-25713, (1992).
Ando et al., "Functions And Modes of Action Of Small G Proteins", Experimental Medicine, vol. 11(15):1873-1979, (1993).
Bengur et al., "Sequence Of The Sites Phosphorylated By Protein Kinase C In The Smooth Muscle Myosin Light Chain", The Journal of Biological Chemistry, vol. 262(16):7613-7617, (1987).
Bradley et al., "Alterations In Cytoplasmic Calcium Sensitivity During Porcine Coronary Artery Contractions As Detected By Aequorin", J. Physiol., vol. 385:437-448, (1987).
Bresnick et al., "Phosphorylation On Threonine-18 Of The Regulatory Light Chain Dissociates The ATPase And Motor Properties Of Smooth Muscle Myosin II", Biochemistry, vol. 34:12576-12583, (1995).
Brook et al., "Molecular Basis of Myotonic Dystrophy: Expansion Of A Trinucleotide (CTG) Repeat At The 3' End Of A Transcript Encoding A Protein Kinase Family Member", Cell, vol. 68:799-808, (1992).
Chen et al., "Molecular Cloning of cDNA Encoding The 110 kDa And 21 kDa Regulatory Subunits Of Smooth Muscle Protein Phosphatase 1M", FEBS Letters, vol. 356:51-55, (1994).
Chong et al., "The Small GTP-Binding Protein Rho Regulates A Phosphatidylinositol 4-Phosphate 5-Kinase In Mammalian Cells", Cell, vol. 79:507-513, (1994).
Collard, "Signaling Pathways Regulated By Rho-like Proteins: A Possible Role In Tumor Formation And Metastasis (Review)", International Journal of Oncology, vol. 8:1-8, (1996).
Drgonova et al., "Rho 1p, A Yeast Protein At The Interface Between Cell Polarization And Morphogenesis", Science, vol. 272:277-279, (1996).
Gong et al., "Role Of Guanine Nucleotide-Binding Proteins-Ras-family Or Trimeric Proteins Or Both-In Ca.sup.2+ Sensitization Of Smooth Muscle", Proc. Natl. Acad. Sci., vol. 93:1340-1345, (1996).
Hart et al., "Cellular Transformation And Guanine Nucleotide Exchange Activity Are Catalyzed By A Common Domain On The dbl Oncogene Product", The Journal of Biological Chemistry, vol. 269(1):62-65, (1994).
Hartshorne, "Biochemistry Of The Contractile Process In Smooth Muscle", Physiology Of The Gastrointestinal Tract,pp. 423-483.
Hathaway et al., "Selective Purification Of The 20,000-Da Light Chains Of Smooth Muscle Myosin", Analytical Biochemistry, vol. 135:37-43, (1983).
Hill et al., "The Rho Family GTPases RhoA, Rac1, And CDC42Hs Regulate Transcriptional Activation By SRF", Cell, vol. 81:1159-1170, (1995).
Hirata et al., "Involvement Of rho p21 In The GTP-Enhanced Calcium Ion Sensitivity Of Smooth Muscle Contraction", The Journal Of Biological Chemistry, vol. 267(13):8719-8722, (1992).
Horii et al., "A Novel Oncogene, ost, Encodes A Guanine Nucleotide Exchange Factor That Potentially Links Rho and Rac Signaling Pathways", The EMBO Journal, vol. 13(20):4776-4786, (1994).
Horiuchi et al., "The Posttranslational Processing Of ras p21 Is Critical For Its Stimulation Of Yeast Adenylate Cyclase", Molecular and Cellular Biology, vol. 12(10):4515-4520, (1992).
Huttenlocher et al., "Adhesion in Cell Migration", Current Opinion In Cell Biology, vol. 7:697-706, (1995).
Ikebe et al., "Phosphorylation Of Smooth Muscle Myosin At Two Distinct Sites By Myosin Light Chain Kinase", The Journal Of Biological Chemistry, vol. 260(18):10027-10031, (1985).
Ikebe et al., "Phosphorylation Of Bovine Platelet Myosin By Protein Kinase C", Biochemistry, vol. 29:2713-2720, (1990).
Ishizaki et al., "The Small GTP-Binding Protein Rho Binds To And Activates A 160 kDa Ser/Thr Protein Kinase Homologous To Myotonic Dystrophy Kinase", The EMBO Journal, vol. 15(8):1885-1893, (1996).
Itoh et al., "The Post-Translational Processing Of ras p21 Is Critical For Its Stimulation Of Mitogen-Activated Protein Kinase", The Journal Of Biological Chemistry, vol. 268(5):3025-3028, (1993).
Kamisoyama et al., "Mutagenesis Of The Phosphorylation Site (Serine 19) Of Smooth Muscle Myosin Regulatory Light Chain And Its Effects On The Properties Of Myosin", Biochemistry, vol. 33:840-847, (1994).
Kamm et al., "The Function of Myosin And Myosin Light Chain Kinase Phosphorylation In Smooth Muscle", Ann. Rev. Pharmacol. Toxicol., vol. 25:593-620, (1985).
Khosravi-Far et al., "Activation Of Rac1, RhoA, And Mitogen-Activated Protein Kinases Is Required For Ras Transformation", Molecular And Cellular Biology, vol. 15(11):6443-6453, (1995).
Kishi et al., "Regulation Of Cytoplasmic Division Of Xenopus Embryo By rho p21 And Its Inhibitory GDP/GTP Exchange Protein (rho GDI)", The Journal of Cell Biology, vol. 120(5):1187-1195, (1993).
Kitazawa et al., "G Protein-Mediated Inhibition Of Myoshin Light-Chain Phosphatase In Vascular Smooth Muscle", Proc. Natl. Acad. Sci. USA, vol. 88:9307-9310, (1991).
Lamarche et al., "GAPs for Rho-Related GTPases", TIG December, vol. 10(12):436-440, (1994).
Laudanna et al., "Role of Rho In Chemoattractant-Activated Leukocyte Adhesion Through Integrins", Science, vol. 271:981-983, (1996).
Lebowitz et al., "Evidence That Farnesyltransferase Inhibitors Suppress Ras Transformation By Interfereing With Rho Activity", Molecular And Cellular Biology, vol. 15(12):6613-6622, (1995).
Leung et al., "A Novel Serine/Threonine Kinase Binding The Ras-Related RhoA GTPase Which Translocates The Kinase To Peripheral Membranes", The Journal of Biological Chemistry, vol. 270(49):29051-29054, (1995).
Mabuchi et al., "A Rho-Like Protein Is Involved In The Organisation Of The Contractile Ring In Dividing Sand Dollar Eggs", Zygote, vol. 1:325-331, (1993).
Mahadevan et al., "Myotonic Dystrophy Mutation: An Unstable CTG Repeat In The 3' Untranslated Region Of The Gene", Science, vol. 255:1253-1255, (1992).
Madaule et al., "A Novel Partner For The GTP-Bound Forms of rho and rac", FEBS Letters, vol. 377:243-248, (1995).
Matsui et al., "Identification Rho Protein Target Protein", Seikagaku, vol. 67(7):649, (1995).
Moe et al., ".beta..sub.2 -Microglobulin Induces Calcium Efflux From Cultured Neonatal Mouse Calvariae", Am. J. Physiol., vol. 263:dF540-F544, (1992).
Morii et al., "A rho Gene Product In Human Blood Platelets", The Journal of Biological Chemistry, vol. 267(29):20921-20926, (1992).
Mukai et al., "A Novel Protein Kinase With Leucine Zipper-Like Sequences: Its Catalytic Domain Is Highly Homologous To That Of Protein Kinase C", Biochemical And Biophysical Research Communications, vol. 199(2):897-904, (1994).
Mukai et al., "Activation Of PKN, A Novel 120-DKa Protein Kinase With Leucine Zipper-Like Sequences, By Unsaturated Fatty Acids And By Limited Proteolysis", Biodhemical And Biophysical Research Communications, vol. 204(1):348-356, (1994).
Nishikawa et al., "Protein Kinase C Modulates in Vitro Phosphorylation Of The Smooth Muscle Heavy Meromyosin By Myosin Light Chain Kinase", The Journal Of Biological Chemistry, vol. 259(14):8808-8814, (1984).
Nishiyama et al., "Regulation of Cytoskeleton By Rho", Experimental Medicine, vol. 12:991-996, (1994).
Noda et al., "Involvement of rho in GTP.gamma.S-induced Enhancement Of Phosphorylation of 20 kDa Myosin Light Chain In Vascular Smooth Muscle Cells: Inhibition Of Phosphatase Activity", FEBS Letters, vol. 367:246-250, (1995).
Nonaka et al., "A Downstream Target of RHO1 Small GTP-Binding Protein Is PKC1, A Homolog Of Protein Kinase C, Which Leads To Activation Of The MAP Kinase Cascade In Saccharomyces Cerevisiae", The EMBO Journal, vol. 14(23):5931-5938, (1995).
Nusrat et al., "Rho Protein Regula

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