Rhamnose-inducible expression constructs and methods

Details Rhamnose-inducible expression constructs and methods Rhamnose-inducible expression constructs and methods

2011-01-18

2011-01-18

Kelly, Robert M (Department: 1633)

Chemistry: molecular biology and microbiology

Vector, per se

C536S023100, C536S024100, C514S04400A

Reexamination Certificate

active

07871815

ABSTRACT:
Disclosed herein is a rhamnose-inducible expression vector.

REFERENCES:
patent: 6274373 (2001-08-01), Virtanen
patent: 6998258 (2006-02-01), Kesseler et al.
patent: 7189506 (2007-03-01), Lim et al.
patent: 2006/0014291 (2006-01-01), Kebeler et al.
patent: WO 03/033519 (2003-04-01), None
Fahlen, et al. (2000) FEMS Immunol. Med. Microbiol., 28(1): 25-35.
Webb, et al. (1999) Methods in Enzymology, 302: 136-53 (PubMed Cite Only).
McNairn, et al. (1995) Molecular Microbiology, 15(3): 507-17 (Abstract Only).
Boyd, et al. (2000) Journal of Bacteriology, 182(3): 842-847.
Giacalone, Matthew J., et al., “Immune responses elicited by bacterial minicells capable of simultaneous DNA and protein antigen delivery,”Vaccine24 (2006) 6009-6017.
Khachatourians, George G., “Minicells as Specialized Vaccines and Vaccine Carriers,” Isaacson, R.E. (Ed.). Recombinant DNA Vaccines: Rationale and Strategy, pp. 323-333, 1992.
Suzuki, Mansanori, et al., “Production inEscherichia coliof biologically active secretin, a gastrointestinal hormone,”Proc. Natl. Acad. Sci. USA, vol. 79, pp. 2475-2479, Apr. 1982.
Supplementary European Search Report dated Oct. 31, 2006.
Barrado, P. et al., Expression inEscherichia coliof a Recombinant Adenosine Kinase fromSaccharomyces cerevisae: Purification, Kenetics and Substrate Analyses, Yeast, 2003, pp. 1145-1150, vol. 20, John Wiley & Sons, Ltd.
de Boer, H. A. et al., Portable Shine-Dalgarno Regions; Nucleotides Between the Shine-Dalgarno Sequence and the Start Codon Affect the Translation Efficiency, Gene Amplification and Analysis, 1983, pp. 103-116, vol. 3.
Egan, S. M. et al., A Regulatory Cascade in the Induction of rhaBAD, Journal of Molecular Biology,1993, pp. 87-98., vol. 234.
Egan, S. M. et al., DNA-Dependent Renaturation of an Insoluble DNA Binding Protein Identification of the RhaS Binding Site at rhaBAD, Journal of Molecular Biology, 1994, pp. 821-829, vol. 243.
Giacalone, M. J. et al., Toxic Protein Expression inEscherichia coliUsing a Rhamnose-based Tightly Regulated and Tunable Promoter System, BioTechniques, Mar. 2006, pp. 355-364, vol. 40, No. 3.
Haldimann, A. et al., Use of New Methods for Construction of Tightly Regulated Arabinose and Rhamnose Promoter Fusions in Studies of theEscherichia coliPhosphate Regulon, Journal of Bacteriology, Mar. 1998, pp. 1277-1286, vol. 180, No. 5, American Society for Microbiology.
Holcroft, C. C. et al., Interdependence of Activation at rhaSR by Cyclic AMP Receptor Protein, the RNA Polymerase Alpha Subunit C-Terminal Domain, and RhaR, Journal of Bacteriolgy, Dec. 2000, pp. 6774-6782, vol. 182, No. 23, American Society for Microbiology.
Moralejo, P. et al., Sequencing and Characterization of a Gene Cluster Encoding the Enzymes for L-Rhamnose Metabolism inEscherichia coli, Journal of Bacteriology, Sep. 1993, pp. 5585-5594, vol. 175, No. 17, American Society for Microbiology.
Tobin, J. F. et al., Positive Regulation of theEscherichia coliL-Rhamnos Operon is Mediated by the Products of Tandemly Repeated Regulatory Genes, Journal of Molecular Biology, 1987, pp. 789-799, vol. 196, Academic Press Limited.
Tobin, J. F. et al., Purification and Properties of RhaR, the Positive Regulator of the L-rhamnose Operons ofEscherichia coli, Journal of Molecular Biology, 1990, pp. 75-89, vol. 211, Academic Press Limited.
Via, P. et al., Transcriptional Regulation of theEscherichia colirhaT Gene. Microbiology, 1996, pp. 1833-1840, vol. 142.
Volff, J.-N. et al., Nucleotide Sequence and Role in DNA Amplification of the Direct Repeats Composing the Amplifiable Element AUD1 ofStreptomyces lividans66, Molecular Microbiology, 1996, pp. 1037-1047, vol. 21, No. 5, Blackwell Science Ltd.
Wickstrum, J. R. et al., Amino Acid Contacts between Sigma 70 Domain 4 and the Transcription Activators RhaS and RhaR, Journal of Bacteriology, Sep. 2004, pp. 6277-6285, vol. 186, No. 18, American Society for Microbiology.
Wickstrum, J. R. et al., Cyclic AMP Recepetor Protein and RhaR Synergistically Activate Transcription from the L-Rhamnose-Responsive rhaSR Promoter inEscherichia coli, Journal of Bacteriology, Oct. 2005, pp. 6708-6718, vol. 187, No. 19, American Society for Microbiology.
Wilms, B. et al., High-Cell-Density Fermentation for Production of L-N-Carbamoylase Using an Expression System Based on theEscherichia colirhaBAD Promoter, Biotechnology and Bioengineering, Apr. 20, 2001, pp. 95-103, vol. 73, No. 2, John Wiley & Sons, Inc.
Stumpp, et al., “En neues, L-Rhamnose-induzierbares Expressionssystem fürEscherichia coli,” Biospektrum, Spektrum Akademischer Verlag, DE, 2000, vol. 6, No. 1, pp. 33-36.

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