Rhamnose binding protein

Details Rhamnose binding protein Rhamnose binding protein

2005-08-16

2005-08-16

Navarro, Mark (Department: 1645)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

C530S380000

Reexamination Certificate

active

06930171

ABSTRACT:
An isolated RBP with at least one of the following characteristics:a) a molecular weight of approximately 65-70 kDa and more preferably 66-69 kDa;b) a pl of greater than 10 or less than 3;c) a dissociation constant of approximately 1.5×10−6when bound to the rhamnose moiety of solamargine;d) adapted to bind to a rhamnose affinity column prepared according to example 1 and under the conditions set out therein;e) adapted to be eluted from the column in example 1 with a 100 mM rhamnose solution;f) insoluble in aqueous solution; andg) soluble in highly denaturing buffers containing greater that approximately 2% surfactant.

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