Reversible immobilization of assay reagents in a multizone test

Details Reversible immobilization of assay reagents in a multizone test Reversible immobilization of assay reagents in a multizone test

1988-10-31

1990-09-25

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

422 56, 422 57, 422 58, 436170, 435805, G01N 3122, G01N 3353

Patent

active

049593056

ABSTRACT:
A multizone test device for the determination of analyte in a liquid test medium. The test device preferably comprises multilayers including a first layer comprising a solid, porous matrix incorporated with a reversibly immobilized first reagent and a second layer comprising a solid, porous matrix incorporated with a second reagent which interacts with the first reagent and analyte from the liquid test medium to provide a detectable signal. A reversible binding interaction between the first reagent and the matrix of the first layer prevents the first reagent from prematurely migrating into the second layer during manufacture of the device and prior to application of the liquid test medium to the test device. The reversible binding interaction is sufficiently disruptable by contact of the first layer with the liquid test medium to release and thereby render diffusible an analytically effective amount of the first reagent within the test device.

REFERENCES:
patent: Re30267 (1981-05-01), Bruschi
patent: 4088538 (1978-05-01), Schneider
patent: 4120945 (1978-10-01), Gutcho et al.
patent: 4144306 (1979-03-01), Figueras
patent: 4258001 (1981-03-01), Pierce et al.
patent: 4277560 (1981-07-01), Gray et al.
Chem. Abstract 89:38969y.

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