Reversible gelling culture media for in-vitro cell culture in th

Details Reversible gelling culture media for in-vitro cell culture in th Reversible gelling culture media for in-vitro cell culture in th

1998-04-17

2000-08-15

Naff, David M.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Solid support and method of culturing cells on said solid...

424 937, 435182, 435396, 435397, 435404, C12N 506, C12N 508, C12N 1104

Patent

active

061035289

ABSTRACT:
A gelling cell culture medium useful for forming a three dimensional matrix for cell culture in vitro is prepared by copolymerizing an acrylamide derivative with a hydrophilic comonomer to form a reversible (preferably thermally reversible) gelling linear random copolymer in the form of a plurality of linear chains having a plurality of molecular weights greater than or equal to a minimum gelling molecular weight cutoff, mixing the copolymer with an aqueous solvent to form a reversible gelling solution and adding a cell culture medium to the gelling solution to form the gelling cell culture medium. Cells such as chondrocytes or hepatocytes are added to the culture medium to form a seeded culture medium, and temperature of the medium is raised to gel the seeded culture medium and form a three dimensional matrix containing the cells. After propagating the cells in the matrix, the cells may be recovered by lowering the temperature to dissolve the matrix and centrifuging.

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