Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1995-06-07
1998-03-10
Chin, Christopher L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
422 55, 422 56, 422 57, 422 58, 422 61, 435 721, 435 722, 435 731, 435 732, 435 736, 435 792, 4352871, 4352872, 4352876, 4352877, 435970, 435810, 436514, 436518, 436528, 436530, 436164, 436169, 436805, 436810, G01N 33569, G01N 33543, G01N 33558
Patent
active
057260133
ABSTRACT:
Disclosed are assay systems, kits, and methods for performing an assay which determines the presence or quantity of an analyte in a fluid sample by detecting binding of the analyte to at least one immobilized analyte capture reagent and washing unbound material from the immobilized analyte capture reagent. An elongated solid phase flow matrix includes: (i) a first region adapted for receipt of the fluid sample, (ii) a second region at which the analyte capture reagent is immobilized, (iii) a third region for application of a liquid wash and/or detector reagent capable of removing unbound substances from the second region; and (iv) an absorbent reservoir of high volume capacity. The second region is positioned between the first region and the third region and between the absorbent reservoir and the third region. The device also includes means to detect analyte bound at the second region. The fluid sample flows by capillary action initially along the elongated flow matrix in one direction toward and through the second region, and subsequently, liquid wash and/or detector reagent flows along the matrix in a second direction opposite the first direction, through the second region, and into the absorbent reservoir, drawing unbound substances with it.
REFERENCES:
patent: 3615222 (1971-10-01), Mead
patent: 3645687 (1972-02-01), Nerenberg
patent: 3715192 (1973-02-01), Wenz et al.
patent: 3799742 (1974-03-01), Coleman
patent: 3811840 (1974-05-01), Bauer et al.
patent: 3825410 (1974-07-01), Bagshawe
patent: 3843324 (1974-10-01), Edelman et al.
patent: 3888629 (1975-06-01), Bagshawe
patent: 3966897 (1976-06-01), Renn et al.
patent: 4039652 (1977-08-01), Adams et al.
patent: 4056284 (1977-11-01), Posch
patent: 4061468 (1977-12-01), Lange et al.
patent: 4088448 (1978-05-01), Lija et al.
patent: 4094647 (1978-06-01), Deutsch et al.
patent: 4125372 (1978-11-01), Kawai et al.
patent: 4138474 (1979-02-01), Updike
patent: 4153675 (1979-05-01), Kleinerman
patent: 4168146 (1979-09-01), Grubb et al.
patent: 4180383 (1979-12-01), Johnson
patent: 4193983 (1980-03-01), Ullman et al.
patent: 4200690 (1980-04-01), Root et al.
patent: 4233029 (1980-11-01), Columbus
patent: 4235601 (1980-11-01), Deutsch et al.
patent: 4246339 (1981-01-01), Cole et al.
patent: 4275149 (1981-06-01), Litman et al.
patent: 4277560 (1981-07-01), Gray et al.
patent: 4298685 (1981-11-01), Parikh et al.
patent: 4305924 (1981-12-01), Piasio et al.
patent: 4318980 (1982-03-01), Boguslaski et al.
patent: 4322495 (1982-03-01), Kato
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4366241 (1982-12-01), Tom et al.
patent: 4376110 (1983-03-01), David et al.
patent: 4391904 (1983-07-01), Litman et al.
patent: 4424279 (1984-01-01), Bohn et al.
patent: 4425438 (1984-01-01), Bauman et al.
patent: 4426451 (1984-01-01), Columbus
patent: 4427769 (1984-01-01), Adlercruetz et al.
patent: 4435504 (1984-03-01), Zuk et al.
patent: 4446869 (1984-05-01), Knodle
patent: 4587102 (1986-05-01), Nagatomo et al.
patent: 4717656 (1988-01-01), Swanljung
patent: 4740468 (1988-04-01), Weng et al.
patent: 4756884 (1988-07-01), Hillman et al.
patent: 4774177 (1988-09-01), Marks
patent: 4826759 (1989-05-01), Guire et al.
patent: 4906439 (1990-03-01), Grenner
patent: 4918025 (1990-04-01), Grenner
patent: 4923680 (1990-05-01), Nelson
patent: 4956302 (1990-09-01), Gordon et al.
patent: 4963498 (1990-10-01), Hillman et al.
patent: 5051237 (1991-09-01), Grenner et al.
patent: 5104793 (1992-04-01), Buck
patent: 5104812 (1992-04-01), Kurn et al.
patent: 5141850 (1992-08-01), Cole et al.
Dafforn et al., Clin. Chem., 36:1312-1316, 1990.
Li et al., Analytical Biochemistry, 166:276-283, 1987.
Zuk et al., Clin. Chem, 31:1144-1150, 1985.
Towbin et al., Journal of Immunological Methods, 72:313-430, 1984.
Pappas et al., Journal of Immunological Methods, 64:205-214, 1983.
Holmberg et al., Journal of Immunological Methods, 61:9-6, 1983.
Jalkanen et al., J. Clin. Lab. Immunol., 10:225-228, 1983.
Hawkes, Analytical Biochemistry, 123:143-146, 1982.
Cleveland et al., Journal of Immunological Methods, 29:369-386, 1979.
Towbin et al., Proc. Natl. Acad. Sci. USA, 76:4350-4354, 1979.
Henning et al., Proc. Natl. Acad. Sci. USA, 76:4360-4364, 1979.
Renart et al., Proc. Natl. Acad. Sci. USA, 76:3116-3120, 1979.
Chin Christopher L.
IDEXX Laboratories, Inc.
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