Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1998-03-09
1999-12-28
Chin, Christopher L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
422 55, 422 56, 422 57, 422 58, 4352871, 4352872, 4352877, 4352876, 4352879, 435810, 435970, 435974, 435975, 436164, 436165, 436169, 436514, 436518, 436528, 436530, 436805, 436807, 436808, 436810, 436811, G01N 33558
Patent
active
060079991
ABSTRACT:
Disclosed are devices and methods for performing an assay which determines the presence or quantity of an analyte in a fluid sample by detecting binding of the analyte to at least one immobilized analyte capture reagent and washing unbound material from the immobilized analyte capture reagent. The devices and methods an elongated solid phase flow matrix, including capillary channels capable of driving capillary fluid movement, and further including (i) a first region adapted for receipt of the fluid sample, (ii) a second region at which the analyte capture reagent is immobilized, (iii) a third region for application of a wash reagent capable of removing unbound substances from the second region; and (iv) an absorbent reservoir of high volume capacity. The second region is positioned intermediate to the first region and the third region and intermediate to the absorbent reservoir and the third region. The device also includes means to detect analyte bound at the second region. The flow matrix and the regions thereof are sized and positioned to cause the fluid sample to flow initially along the elongated flow matrix in one direction toward and through the second region, and subsequently, the liquid reagent to flow along the elongated flow matrix in a second direction opposite the first direction, through the second region, and into the absorbent reservoir, drawing unbound substances with it.
REFERENCES:
patent: 3615222 (1971-10-01), Mead
patent: 3645687 (1972-02-01), Nerenberg
patent: 3715192 (1973-02-01), Wenz et al.
patent: 3799742 (1974-03-01), Coleman
patent: 3811840 (1974-05-01), Bauer et al.
patent: 3825410 (1974-07-01), Bagshawe
patent: 3843324 (1974-10-01), Edelman et al.
patent: 3888629 (1975-06-01), Bagshawe
patent: 3966897 (1976-06-01), Renn et al.
patent: 4039652 (1977-08-01), Adams et al.
patent: 4053284 (1977-10-01), Posch
patent: 4061468 (1977-12-01), Lange et al.
patent: 4088448 (1978-05-01), Lija et al.
patent: 4094647 (1978-06-01), Deutsch et al.
patent: 4125372 (1978-11-01), Kawai et al.
patent: 4138474 (1979-02-01), Updike
patent: 4153675 (1979-05-01), Kleinerman
patent: 4168146 (1979-09-01), Grubb et al.
patent: 4180383 (1979-12-01), Johnson
patent: 4193983 (1980-03-01), Ullman et al.
patent: 4200690 (1980-04-01), Root et al.
patent: 4233029 (1980-11-01), Columbus
patent: 4235601 (1980-11-01), Deutsch et al.
patent: 4246339 (1981-01-01), Cole et al.
patent: 4275149 (1981-06-01), Litman et al.
patent: 4277560 (1981-07-01), Gray et al.
patent: 4298685 (1981-11-01), Parikh et al.
patent: 4305924 (1981-12-01), Piasio et al.
patent: 4318980 (1982-03-01), Boguslaski et al.
patent: 4322495 (1982-03-01), Kato
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4366241 (1982-12-01), Tom et al.
patent: 4376110 (1983-03-01), David et al.
patent: 4391904 (1983-07-01), Litman et al.
patent: 4424279 (1984-01-01), Bohn et al.
patent: 4425438 (1984-01-01), Bauman et al.
patent: 4426451 (1984-01-01), Columbus
patent: 4427769 (1984-01-01), Adlercruetz et al.
patent: 4435504 (1984-03-01), Zuk et al.
patent: 4446869 (1984-05-01), Knodle
patent: 4587102 (1986-05-01), Nagatomo et al.
patent: 4717656 (1988-01-01), Swanljung
patent: 4740468 (1988-04-01), Weng et al.
patent: 4756884 (1988-07-01), Hillman et al.
patent: 4774177 (1988-09-01), Marks
patent: 4826759 (1989-05-01), Guire et al.
patent: 4839275 (1989-06-01), Weil
patent: 4906439 (1990-03-01), Grenner
patent: 4918025 (1990-04-01), Grenner
patent: 4923680 (1990-05-01), Nelson
patent: 4956302 (1990-09-01), Gordon et al.
patent: 4963498 (1990-10-01), Hillman et al.
patent: 5051237 (1991-09-01), Grenner et al.
patent: 5073484 (1991-12-01), Swanson et al.
patent: 5104793 (1992-04-01), Buck
patent: 5104812 (1992-04-01), Kurn et al.
patent: 5141850 (1992-08-01), Cole et al.
patent: 5468648 (1995-11-01), Chandler
patent: 5654162 (1997-08-01), Guire et al.
patent: 5726010 (1998-03-01), Clark
patent: 5726013 (1998-03-01), Clark
patent: 5750333 (1998-05-01), Clark
Dafforn et al., Clin. Chem., 36:1312-1316, 1990.
Li et al., Analytical Biochemistry, 166:276-283, 1987.
Zuk et al., Clin. Chem, 31:1144-1150, 1985.
Towbin et al., Journal of Immunologial Methods, 72:313-430, 1984.
Pappas et al., Journal of Immunological Methods, 64:205-214, 1983.
Holmberg et al., Journal of Immunological Methods, 61:9-6, 1983.
Jalkanen et al., J. Clin. Lab. Immunol., 10:225-228, 1983.
Hawkes, Analytical Biochemistry, 123:143-146, 1982.
Cleveland et al., Journal of Immunological Methods, 29:369-286, 1979.
Towbin et al., Proc. Natl. Acad. Sci. USA, 76:4350-4354, 1979.
Henning et al., Proc. Natl Acad. Sci. USA, 76:4360-4364, 1979.
Renart et al., Proc. Natl. Acad. Sci. USA, 76:3116-3120, 1979.
Chin Christopher L.
IDEXX Laboratories, Inc.
LandOfFree
Reversible flow chromatographic binding assay does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Reversible flow chromatographic binding assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Reversible flow chromatographic binding assay will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2381419