Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1987-05-19
1989-03-14
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 34, 436519, 436520, 436526, C12Q 102
Patent
active
048124010
ABSTRACT:
Compounds and methods are disclosed for reversibly aggregating particles suspended in a liquid medium. The method comprises combining the liquid medium containing the particles with a polyionic polymer capable of aggregating the particles under conditions suitable for such aggregation. Thereafter, the particles are contacted with a chemical reagent capable of cleaving the polyionic polymer under conditions sufficient to reverse the aggregation. Optionally, magnetic particles are added to the liquid medium in the present method under conditions for non-specific binding and the medium including the aggregates is subjected to a magnetic field gradient to separate the aggregates from the medium. The compounds of the present invention are polyions. The aggregation of the particles is reversible upon contact with chemical agents which cleave at least some of the bonds within the polyionic polymer.
REFERENCES:
patent: 3970518 (1976-07-01), Giaever
patent: 4230685 (1980-10-01), Senyei
patent: 4369226 (1983-01-01), Rembaum
patent: 4452773 (1984-06-01), Molday
patent: 4687748 (1987-08-01), Schroder
Lin Cheng-I
Tarnowski Thomas L.
Ullman Edwin F.
Barrett Carole F.
English Debra
Leitereg Theodore J.
Marantz Sidney
Syntex (U.S.A.) Inc.
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