Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-05-12
1994-12-06
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 4, 435 725, 436519, 436520, 436526, 436531, 436534, 436808, 436829, 210222, 210223, 210695, 210704, 210705, 210710, 209 5, 209214, G01N 3353
Patent
active
053709934
ABSTRACT:
Compounds and methods are disclosed for reversibly aggregating particles suspended in a liquid medium. The method comprises combining the liquid medium containing the particles with a polyionic polymer capable of aggregating the particles under conditions suitable for such aggregation. Thereafter, the particles are contacted with a chemical reagent capable of cleaving the polyionic polymer under conditions sufficient to reverse the aggregation. Optionally, magnetic particles are added to the liquid medium in the present method under conditions for non-specific binding and the medium including the aggregates is subjected to a magnetic field gradient to separate the aggregates from the medium. The compounds of the present invention are polyions. The aggregation of the particles is reversible upon contact with chemical agents which cleave at least some of the bonds within the polyionic polymer.
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Lin Cheng-I
Tarnowski Thomas L.
Ullman Edwin F.
Barrett Carole F.
Bosse Mark L.
Kepplinger Esther M.
Precivale Shelley G.
Syntex (U.S.A.) Inc.
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