Reverse two-hybrid systems

Details Reverse two-hybrid systems Reverse two-hybrid systems

2006-02-13

2009-10-13

Vogel, Nancy (Department: 1636)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C435S254200, C435S006120

Reexamination Certificate

active

07601533

ABSTRACT:
Disclosed are methods for identifying molecular interactions (e.g., protein/protein, protein/DNA, protein/RNA, or RNA/RNA interactions). All of the methods within the invention employ counterselection and at least two hybrid molecules. Molecules which interact reconstitute a transcription factor and direct expression of a reporter gene, the expression of which is then assayed. Also disclosed are genetic constructs which are useful in practicing the methods of the invention.

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Mendelsohn, A.R. and Bre

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