Reverse two-hybrid systems

Details Reverse two-hybrid systems Reverse two-hybrid systems

2006-04-25

2006-04-25

Guzo, David (Department: 1636)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S006120, C435S007200, C435S007310, C435S029000, C435S034000

Reexamination Certificate

active

07033768

ABSTRACT:
Disclosed are methods for identifying molecular interactions (e.g., protein/protein, protein/DNA, protein/RNA, or RNA/RNA interactions). All of the methods within the invention employ counterselection and at least two hybrid molecules. Molecules which interact reconstitute a transcription factor and direct expression of a reporter gene, the expression of which is then assayed. Also disclosed are genetic constructs which are useful in practicing the methods of the invention.

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