Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-10-24
1999-10-12
Elliott, George C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
4352542, 4353201, C12Q 168, C12N 119, C12N 1581
Patent
active
059653680
ABSTRACT:
Disclosed are methods for identifying molecular interactions (e.g., protein/protein, protein/DNA, protein/RNA, or RNA/RNA interactions). All of the methods within the invention employ counterselection and at least two hybrid molecules. Molecules which interact reconstitute a transcription factor and direct expression of a reporter gene, the expression of which is then assayed. Also disclosed are genetic constructs which are useful in practicing the methods of the invention.
REFERENCES:
patent: 5283173 (1994-02-01), Fields et al.
patent: 5695941 (1997-12-01), Brent et al.
Alani et al., "A Method for Gene Disruption That Allows Repeated Use of URA3 Selection in the Construction of Multiply Disrupted Yeast Strains", Genetics, 116:541-545, 1987.
Bartel et al., "BioFeedback", BioTechniques, 14:920-924, 1993.
Baudin et al., "A Simple and Efficient Method for Direct Gene Deletion in Saccharomyces Cerevisae", Nucleic Acids Research, 21:3329-3330, 1993.
Berger et al., "Genetic Isolation of ADA2: A Potential Transcriptional Adaptor Required for Function of Certain Acidic Activation Domains", Cell, 70:251-265, 1992.
Boeke et al., "A Positive Selection for Mutants Lacking Orotindine-5'-Phosphate Decarboxylase Activity In Yeast: 5-Fluoro-Orotic Acid Resistance", Mol Gen Genet, 197:345-346, 1984.
George Boguslawski, "Effects of Polymyxin B Sulfate and Polymyxin B Nonapeptide On Growth and Permeability of the Yeast Saccharomyces Cerevisiae", Mol. Gen. Genet, 199:401-405, 1985.
George Boguslawski, "Polymyxin B Nonapeptide Inhibits Mating in Saccharomyces Cerevisiae" Antimicrobial Agents and Chemotherapy, 29:330-332, 1986.
Buckingham et al., "Nucleotide Sequence and Promoter Analysis of SPO13, a Meiosis-specific Gene of Saccharomyces Cerevisiae", Proc. Natl. Acad. Sci., 87:9406-9410, 1990.
Chalfie et al., "Green Fluorescent Protein as a Marker for Gene Expression", Science, 263:802-805, 1994.
Chevray et al., "Protein Interaction Cloning in Yeast: Identification of Mammalian Proteins that React with the Leucine Zipper of Jun", Proc. Natl. Acad. Sci., 89:5789-5793, 1992.
Chien et al., "The Two-Hybird System: A Method to Identify and Clone Genes for Proteins that Interact with Protein of Interest", Proc. Natl. Acad. Sci., 88:9578-9582, 1991.
Fields et al., "a Novel Genetic System to Detect Protein-Protein Interactions", Nature, 340:245-246, 1989.
Ingles et al., "Reduced Binding of TFIID to Transcriptionally Compromised Mutants of VP16", Nature, 351:588-590, 1991.
Durfee et al., "The Retinoblastoma Protein Associates with the Protein Phosphatase Type 1 Catalytic Subunit", Genes & Development, 7:555-569, 1993.
Helin, et al., "Inhibition of E2F-1 Transactivation by Direct Binding of the Retinoblastoma Protein", Molecular and Cellular Biology, 13:6501-6508, 1993.
Hiebert et al., "The Interaction of RB with E2F Coincides with an Inhibition of the Transcriptional Activity of E2F", Genes & Development, 6:177-185, 1992.
Hope et al., "Functional Dissection of a Eukaryotic Transcriptional Activator Protein, GCN4 of Yeast", Cell, 46:885-894, 1986.
Kumar et al., "Functional Domains of the Human Estrogen Receptor", Cell, 51:941-951, 1987.
Lillie et al., Transcription Activation by the Adenovirus E1a Protein, Nature, 338:39-44, 1989.
Luche et al., "A Cis-cting Element Present in Multiple Genes Serves as a Repressor Protein Binding Site for the Yeast CARI Gene", Molecular and Cellular Biology, 10:3884-3895, 1990.
Ma et al., "A New Class of Yeast Transcriptional Activators", Cell, 51:113-119, 1987.
Pierrat et al., "Functional Analysis of the Human Estrogen Receptor Using a Phenotypic Transactivation Assay in Yeast", Gene, 119:237-245, 1992.
Muhlrad et al., "A Rapid Method for Localized Mutagenesis of Yeast Genes", Yeast, 8:79-82, 1992.
Rose et al., "Structure and Function of the Yeast URA3 Gene: Expression in Escherichia Coli", Gene, 29:113-124, 1984.
Rose et al., "Identification of a Ty Insertion Within the Coding Sequence of the S. Cerevisiae URA3 Gene", Mol. Gen. Genet, 193:557-560, 1984.
Rodney J. Rothstein, "One Step Gene Disruption in Yeast", Methods in Enzymology, 101:202-211, 1983.
Sardet et al., "E2F-4 and E2F-5, Two Members of the E2F Family, are Expressed in the Early Phases of the Cell Cycle", Proc. Natl. Acad. Sci., 92:2403-2407, 1995.
Sikorski et al., "A System of Shuttle Vectors and Yeast Host Strains Designed for Efficient Manipulation of DNA in Saccharomyces Cerevisiae", Genetics, 122:19-27, 1989.
Sikorski et al., "In Vitro Mutagenesis and Plasmid Shuffling: From Cloned Gene to Mutant Yeast", Methods In Enzymology, 194:302-319, 1991.
Strich et al., "UME6 is a Key Regulator of Nitrogen Repression and Meiotic Development", Genes & Development, 8:796-810, 1994.
Vidal et al., "Identification of Essential Nucleotides in an Upstream Repressing Sequence of Saccharomyces Cerevisiae by Selection for Increased Expression of TRK2", Proc. Natl. Acad. Sci., 92:2370-2374, 1995.
Vidal et al., "RPDI (SIN3/UME4) Is Required for Maximal Activation and Repression of Diverse Yeast Genes", Molecular and Cellular Biology, 11:6306-6316, 1991.
Whyte et al., "Association Between an Oncogene and an Anti-oncogene: The Adenovirus E1A Proteins Bind to the Retinoblastoma Gene Product", Nature, 334:124-129, 1988.
Yang et al., "Protein-peptide Interactions Analyzed with the Yeast Two-hybrid System", Nucleic Acid Research, 23:1152-1156, 1995.
Le Douarin et al., "A New Version of the Two-Hybrid Assay For Detection of Protein-protein Interactions," Mar. 11, 1995, Nucl. Acids Res. 23:876-878.
C.A. Leanna et al., "The Reverse Two-Hybrid System: A Genetic Scheme for Selection Against Specific Protein/ Protein Interactions", 1996 Oxford University Press, Nucleic Acids Research, 1996, vol. 24, No. 17, 3341-3347.
D.J. SenGupta et al., "A Three-Hybrid System to Detect RNA-Protein Interactions In Vivo", Genetics, vol. 93, pp. 8496-8501, Aug. 1996.
S.L. Mansour et al., "Disruption of the Proto-Oncogene int-2 in Mouse Embryo-Derived Stem Cells: A General Strategy for Targeting Mutations to Non-Selectable Genes", Nature, vol. 336, Nov. 24, 1985, pp. 347-352.
Hai-Ling Hsu et al., "Formation of In Vitro Complexes Between the TAL1 and E2A Polypeptides of Leukemic T Cells", Proc. Natl. Acad. Sci. USA, Medical Sciences, vol. 91, Apr. 1994, pp. 181-3185.
Benton et al. (1990) Mol. Cell. Biol. 10:353-360.
Mendelsohn et al. (1994) Curr. Opin. Biotechnol. 5:482-486.
Bendixen et al. (1994) Nucleic Acids Res. 22:1778-1779.
Li et al. (1993) Science 262:1870-1874.
Boeke et al. (1987) Methods Enzymol. 154:164-175.
Boeke Jef D.
Harlow Ed
Vidal Marc
Elliott George C.
Schwartzman Robert
The General Hospital Corporation
The Johns Hopkins University
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