Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-08-04
1999-09-07
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 71, 435 29, 435 697, 435 698, 435455, 435465, 435483, 536 234, C12Q 168, C12N 1563, C12N 510, C12N 1511
Patent
active
059486201
ABSTRACT:
The invention relates to modified two-hybrid systems, in particular a reverse two-hybrid system which employs as a reporter a gene encoding a modifying agent such as an enzyme, and a signal agent which is modified by the enzyme usually by being broken down, such that in the event of an inhibition of binding of the two hybrid proteins a detectable signal is produced. The system is particularly useful for drug screening.
REFERENCES:
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patent: 5885779 (1999-03-01), Sadowski et al.
E. Baum et al. ".beta.-Galactosidase containing a human immunodeficiency virus protease cleavage site is cleaved and inactivated by human immunodificiency virus protease", Proc. Natl. Acad. Sci., vol. 87, pp. 10023-10027, Dec. 1990.
U. Yavuzer et al., "pWITCH: a versatible two-hybrid assay vector for the production of epitope/activation domain-tagged proteins both in vitro and in yeast", Gene, vol. 165, pp. 93-96, 1995.
Dale Susan Jane
Fallon Rachel Alison
Hurd Douglas
Workman Nicholas Ian
Amersham International plc
Horlick Kenneth R.
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