Reverse transcription with thermostable DNA polymerases - high t

Details Reverse transcription with thermostable DNA polymerases - high t Reverse transcription with thermostable DNA polymerases - high t

1989-12-22

1994-06-21

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 9151, 435 791, 435 912, 435 9121, 536 167, 536 256, 935 77, 935 73, C12P 1934, C12Q 168, C07H 504, C07H 1512

Patent

active

053227700

ABSTRACT:
Methods are provided for the replication and amplification of RNA sequences by thermostable DNA polymerases. Reverse transcription of RNA is catalyzed by, for example, 92 kDa Taq, 64 kDa Taq, and Tth DNA polymerase. Reverse transcription is coupled to PCR amplification in a one enzyme procedure using a thermostable polymerase.

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