Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-06-24
1994-05-10
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 91, 435 791, 536 187, 536 256, 935 77, 935 78, C12P 1934, C12Q 168, C07H 504, C07H 1512
Patent
active
053106520
ABSTRACT:
Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided.
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Gelfand David H.
Myers Thomas W.
Escallon Miguel H.
Gould George M.
Hoffman-La Roche Inc.
Sias Stacey R.
Tramaloni Dennis P.
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