Reverse transcription method

Details Reverse transcription method Reverse transcription method

1997-04-21

2000-02-29

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 911, 435 9121, 536 221, 536 231, C12P 1934

Patent

active

060308141

ABSTRACT:
A method of improving the synthesis of full-length cDNA transcripts by Mn.sup.++ -dependent reverse transcriptases, preferably DNA-dependent DNA polymerases, is disclosed.

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N. Baskaran, et al., "Uniform Amplification of a Mixture of Deoxyribonucleic Acids with Varying GC Content," Genome Research 6:633-638, 1996.
D.S. Mytelka and M.J. Chamberlin, "Analysis and suppression of DNA polymerase pauses associated with a trinucleotide consensus," Nucl. Acids Res. 24(14):2774-2781, 1996.
W.A. Rees, et al., "Betaine Can Eliminate the Base Pair Composition Dependence of DNA Melting," Biochemistry 32(1):137-144, 1993.
T. Weissensteiner and J.S. Lanchbury, "Strategy for Controlling Preferential Amplification and Avoiding False Negatives in PCR Typing," BioTechniques 21(6):1102-1108, 1996.

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