Reverse transcriptase from human milk, method for its purificati

Details Reverse transcriptase from human milk, method for its purificati Reverse transcriptase from human milk, method for its purificati

1980-04-28

1983-10-11

Nucker, Christine M.

Chemistry: analytical and immunological testing

Involving diffusion or migration of antigen or antibody

Through a gel

435 7, 435 15, 436530, 436531, 436542, G01N 3356, G01N 3358

Patent

active

044092008

ABSTRACT:
A method for diagnosing mammalian breast cancer by detecting in the physiological fluid of said mammal an antigen (ACRT) having immune cross-reactivity with human reverse transcriptase, or detecting antibodies against said ACRT or detecting antibody-ACRT complexes, wherein the reverse transcriptase is substantially purified, has a molecular of about 70,000 and a sedimentation coefficient on a glycerol gradient of between 5 and 5.5 S. A process for the purification of reverse transcriptase from human milk.

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Gerard et al., Nature, vol. 256, Jul. 10, 1975, pp. 140-143.
Judith A. Kantor et al., "DNA Polymerase with Characteristics of Reverse Transcriptase Purified from Human Milk", Science 5/4/79, v. 204, pp. 511-513.
T. Ohno et al., "Purification and Characterization of the DNA Polymerase of Human Breast Cancer Particles", Proc. Natl. Acad. Sci. U.S.A. 74 (1977), 764-768.

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