Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1994-07-27
1997-05-06
LeGuyader, John L.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 691, 4351721, 4353201, 435325, 435357, C12N 510, C12N 1510
Patent
active
056270585
ABSTRACT:
Retroviruses are used as genetic tools to isolate transcriptionally active chromosomal regions. The retroviruses have a promoterless protein coding sequence located in U3 or U5. The retroviruses may be used to infect cells under conditions which permit selection for instances when the retrovirus integrates in close proximity to and under the control of a cellular promoter. The promoter and its associated gene then may be identified and isolated. In this manner, the retroviruses function as promoter-traps. Related methods and products including vectors, kits and assays are provided.
REFERENCES:
Casadaban et al., "Lactose Genes Fused to Exogenous Promoters in One Step Using a Mu-Lac Bacteriophage: In vivo Probe for Transcriptional Control Sequences", PNAS, vol. 76, No. 9, pp. 4530-4533, Sep. 1979.
Brenner et al., "Analysis of Mammolian Cell Genetic Regulation in situ by Using Retrovirus-Derived Portable Exons Carrying the Escherichia coli Lac Z Gene", PNAS, vol. 86, pp. 5517-5521, Jul. 1989.
Ruley H. Earl
von Melchner Harold
Guzo David
LeGuyader John L.
Massachusetts Institute of Technology
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