Retrovirus promoter-trap vectors

Details Retrovirus promoter-trap vectors Retrovirus promoter-trap vectors

1990-05-14

1994-11-15

Walsh, Stephen G.

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

4351723, 536 231, 536 2433, 935 32, C12N 701, C12N 1511, C12N 1565

Patent

active

053647831

ABSTRACT:
Retroviruses are used as genetic tools to isolate transcriptionally active chromosomal regions. The retroviruses have a promoterless protein coding sequence located in U3 or U5. The retroviruses may be used to infect cells under conditions which permit selection for instances when the retrovirus integrates in close proximity to and under the control of a cellular promoter. The promoter and its associated gene then may be identified and isolated. In this manner, the retroviruses function as promoter-traps. Related methods and products including vectors, kits and assays provided.

REFERENCES:
patent: 4738922 (1988-04-01), Haseltine et al.
patent: 4952499 (1990-08-01), Cantor et al.
patent: 4980289 (1990-12-01), Temin et al.
patent: 4999421 (1991-03-01), Brunck et al.
von Melchner et al., "Identification of Cellular Promoters", J. Virology 63: 3227-3233, Aug. 1989.
Lobel et al., "Construction and Recovery of Viable Retroviral Gemomes . . . ", Science 228: 329-332, 19 Apr. 1985.
Reik et al., "Replication-competent Maloney murine leukemia virus . . . " PNAS 82:1141-1145, Feb. 1985.
von Melchner et al. 1990. Proc. Natl. Acad. Sci. USA 87,3733-3737. Dougherty et al. 1987. Proc. Natl. Acad Sci USA. 84,1197-1201.
Hiller et al. 1988 Molec. Cellular Bio. 8,3298-3302 Murphy et al. 1989. J. Virol. 63,319-327.
Kucherlapati et al. 1988, J. Cell. Biochem. Supp 12B. 0. 198, #H306.

Affiliated with

Also associated with

Rating

Retrovirus promoter-trap vectors does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Retrovirus promoter-trap vectors, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Retrovirus promoter-trap vectors will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1097037