Chemistry: molecular biology and microbiology – Vector – per se
Patent
1993-06-11
1997-05-20
Fleisher, Mindy
Chemistry: molecular biology and microbiology
Vector, per se
435 691, 4351723, C12N 1564, C12N 1568, C12N 1585, C12N 1586
Patent
active
056311621
ABSTRACT:
A process and means for the design and the optimization of retroviral vectors transducing human .beta.-globin gene and .beta.-Locus Control Region (.beta.-LCR) derivatives, hereafter referred to as [.beta.-globin/LCR] retroviruses, which successfully meet the following criteria required for gene therapy applications: (1) stability of proviral transmission (low frequency of rearrangements similar to retroviral vectors considered stable in the art) upon infection of cell-lines and murine bone marrow cells, (2) improved viral titer, thereby allowing successful infection of bone marrow cells, and (3) high erythroid expression of the transduced human .beta.-globin gene, are described, along with specific constructs.
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Leboulch Philippe
London Irving M.
Tuan Dorothy
Degen Nancy J.
Fleisher Mindy
Massachusetts Institute of Technology
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