Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2007-12-04
2007-12-04
Paras, Jr., Peter (Department: 1632)
Chemistry: molecular biology and microbiology
Vector, per se
C514S04400A
Reexamination Certificate
active
10836806
ABSTRACT:
A retroviral vector is described. The retroviral vector comprises a functional splice donor site and a functional splice acceptor site; wherein the functional splice donor site and the functional splice acceptor site flank a first nucleotide sequence of interest (“NOI”); wherein the functional splice donor site is upstream of the functional splice acceptor site; wherein the retroviral vector is derived from a retroviral pro-vector; wherein the retroviral pro-vector comprises a first nucleotide sequence (“NS”) capable of yielding the functional splice donor site and a second NS capable of yielding the functional splice acceptor site; wherein the first NS is downstream of the second NS; such that the retroviral vector is formed as a result of reverse transcription of the retroviral pro-vector.
REFERENCES:
patent: 6544771 (2003-04-01), Riviere et al.
patent: 94/29470 (1994-12-01), None
patent: 98/15636 (1996-04-01), None
patent: 96/28563 (1996-09-01), None
Morgenstern J.P., et al., Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line, Nucleic Acids Research, vol. 18, No. 12, pp. 3587-3596 (May 7, 1990).
Bilbao, G. et al., Adenoviral/retroviral vector chimeras: a novel strategy to achieve high-efficiency stable transduction in vivo, The FASEB Journal, vol. 11, pp. 624-637 (Jul. 11, 1997).
Promega Product Catalog, “Mammalian Expression”, pp. 254 and 255 (1997).
Zheng, B., et al., “Increment of hFIX expression with endogenous intron 1 in vitro1”, Cell Research, vol. 7, No. 1, pp. 21-29 (Jun. 1997).
Constance L. Cepko et al., “Construction and Applications of a Highly Transmissible Murine Retrovirus Shuttle Vector.”, Cell, vol. 37, pp. 1053-1062, 1984.
Glenn Dranoff et al., “Vaccination with irradiated tumor cells engineered to secrete murine granulocyte-macrophage colony-stimulating factor stimulates potent, specific and long-lasting anti-tumor immunity.”, Proceedings of the National Academy of Science, vol. 90, pp. 3539-3543, 1993.
W. J. Krall et al., “Increased levels of spliced RNA account for augmented expression from the MPG retroviral vector in hematopoietic cells.”, Gene Therapy, vol. 3, pp. 37-48, 1996.
Frank Lee et al., “Glucocorticoids regulate expression of dihydrofolate reductase cDNA in mouse mammary tumour virus chimaeric plasmids.”, Nature, vol. 294, pp. 228-232, 1981.
John A. Lewis, “Structure and Expression of the Chinese Hamster Thymidine Kinase Gene.”, Molecular and Cellular Biology, vol. 6, pp. 1998-2010, 1986.
Barbara S. Chapman et al., “Effect of intron A from human cytomegalovirus (Towne) immediate-early gene on heterologous expression in mammalian cells.”, Nucleic Acids Research vol. 19, No. 14, pp. 3979-3986, 1991.
Ismail et al. split intron retroviral vectors: enhanced expression with improved safety vol. 74, No. 5 p. 2365-2371, 2000.
Kriegler et al. Transformation mediated by the SV40 T antigens: separation of the overlapping SV40 early genes with a retroviral vector vol. 38, p. 483-491, Sep. 1984.
Takeda et al, Construction of Chimaeric processed immunoglobulingenes containing mouse variable and human constant region sequences vol. 314 Apr. 1985.
Bebbington Christopher Robert
Kingsman Alan John
Kingsman Susan Mary
Mitrophanos Kyriacos
Uden Mark
Frommer & Lawrence & Haug LLP
Kowalski Thomas J.
Noble Marcia S.
Oxford Biomedica (UK) Limited
Paras, Jr. Peter
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