Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Patent
1994-11-17
1997-09-30
Guzo, David
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
4353201, 435357, C12N 510, C12N 1579
Patent
active
056725108
ABSTRACT:
A retroviral vector including a multiple cloning site having no greater than about 70 base pairs, and which includes at least four different enzyme restriction sites, wherein at least two of the sites have an average frequency of appearance in eukaryotic genes of less than one in 10,000 base pairs. Such vector may be employed in conjunction with a shuttle cloning vector having complementary cloning sites to accomplish transfers of genes and/or promoters between the shuttle cloning vector and the retroviral vector. Such a system provides for efficient transfer of genes and/or promoters to a retroviral vector without necessitating reconstruction of the entire retroviral vector. Also contemplated within the scope of the present invention is a retroviral vector having a 3' LTR wherein at least the promoter sequence of the 3' LTR is mutated such that the promoter sequence becomes nonfunctional.
REFERENCES:
Yu, et al., Proc. Nat. Acad. Sci. USA, vol. 83, pp. 3194-3198 (May 1986).
Davison, et al., Methods in Enzymology, Wu, et al., eds, vol. 153 pp. 34-54 (1987).
Miller, et al., Bio Techniques, vol. 7, No. 9, pp. 980-990 (1989).
Palmer, et al., Blood, vol. 73, No. 2, pp. 438-445 (Feb. 1989).
Idzerda, et al., Mol. Cell. Biol., pp. 5154-5162 (Nov. 1989).
Quitschke, et al., J. Biol. Chem., vol. 284, No. 16, pp. 9539-9546 (1989).
Anderson W. French
Eglitis Martin
Thompson J. Anthony
Genetic Therapy, Inc.
Guzo David
Lillie Raymond J.
Olstein Elliot M.
The United States of America as represented by the Secretary Dep
LandOfFree
Retroviral vectors does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Retroviral vectors, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Retroviral vectors will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2256172