Retroviral vector particles for transducing non-proliferating ce

Details Retroviral vector particles for transducing non-proliferating ce Retroviral vector particles for transducing non-proliferating ce

1994-01-14

1996-11-19

Guzo, David

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4352402, 4353201, 435 691, 4351723, 4352351, 536 231, 536 234, 536 2372, 536 241, C12N 1586, C12N 510, C12P 2100, C07H 2104

Patent

active

055762018

ABSTRACT:
Retroviral vector particles are provided which contain: 1) oncoretroviral gag, pol, and env proteins, including an oncoretroviral gag matrix protein which has been mutated so as to contain a nuclear localization signal (NLS) sequence; and 2) at least one exogenous gene. The particles can be used to transduce non-proliferating cells, including stem cells and neurons. The presence of the NLS sequence allows the at least one exogenous gene to enter into the nucleus of a target cell, thus allowing integration of the gene into the genome of the target cell.

REFERENCES:
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