Retroviral vector, a replication system for said vector and avia

Details Retroviral vector, a replication system for said vector and avia Retroviral vector, a replication system for said vector and avia

1996-11-15

1999-03-23

Degen, Nancy

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 691, 4351721, 435325, 435349, 4353201, C12N 1585, C12N 1511, C07H 2104

Patent

active

058861661

ABSTRACT:
A retroviral vector comprising a retrovirus with infectivity for birds and/or mammals in which at least part of the genomic RNA sequences carrying information for the production of viral proteins required in trans for retroviral replication have been replaced by one or more sequences carrying information to be introduced in a target cell chromosome, characterized in that the primer binding site (PBS) has been modified to a sequence that does not allow strong base pairing with the 3' end or other priming sequence in any naturally occurring tRNA. The present application is directed to the tRNA-like primer.

REFERENCES:
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Pedersen, F.S. et al., "Control Points for Retroviral Vector Safety: A Look at the MLV Replication Cycle", abstract of paper presented at Goslar, Germany, conference Sep. 18, 1994.
Lund et al., Journal of Virology, Feb. 1997, Vol. 71, pp 1191-1195.
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Atze et al "Reduced Replication of Human Immunodeficidency type 1 Mutants That Use Reverse Transcription primers other than tRNA .sub.3.sup.Lys ". J Virology vol. 69(5), pp. 309-3097, 1995.
Weiss et al. "Synthetic human tRNa.sub.wv .sup.lys and natural bovine tRNA.sub.suu .sup.Lys3 u u u.sup.Lys3 interact with HIV-1 reverse transcriptase and serve as specific primers for retroviral cDNA synthesis. " GENE vol III:183-197, 1992.
Lund et al., "Mutated Printer Binding Sites Interacting with Different tRNAs allow Efficient Murine Leukemia Virus Replication" J. of Virology vol. 67(12):7125-7130, 1993.
Kreg et al., "the use of synthetic tRNA gene as a novel approach to study in vivo transcription and chromatin structure in yeast". NAR vol.19(4):3849-3855, 1991.
Barat et al., "Interaction of HIV-1 reverse transcriptse with a synthetic form of its replication primr, TRNA .sup.Lys,3 ". NAR vol. 19(4):751-757, 1991.

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