Retroviral vector, a replication system for said vector and avia

Details Retroviral vector, a replication system for said vector and avia Retroviral vector, a replication system for said vector and avia

1995-09-08

1999-02-02

Degen, Nancy

Chemistry: molecular biology and microbiology

Vector, per se

435 691, 4351721, 435325, 435349, 536 2433, C12N 1585, C12N 1511, C07H 2104

Patent

active

058664114

ABSTRACT:
A retroviral vector comprising a retrovirus with infectivity for birds and/or mammals in which at least part of the genomic RNA sequences carrying information for the production of viral proteins required in trans for retroviral replication have been replaced by one or more sequences carrying information to be introduced in a target cell chromosome, characterized in that the primer binding site (PBS) has been modified to a sequence that does not allow strong base pairing with the 3' end or other priming sequence in any naturally occurring tRNA.

REFERENCES:
Pedersen, F.S. et al., "Control Points for Retrovral Vector Safety: A Look at the MLV Replication Cycle", abstract of paper presented at Goslar, Germany, conference Sep. 18, 1994.
Lund et al., Journal of Virology, Feb. 1997, vol. 71, pp. 1191-1195.
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Isel et al., J. Mol. Biol. (1995) 247, pp. 236-250.
Barat, C., V. Lullien, O. Schatz, G. Keith, M.T. Nugeyre, F. Gruninger-Leitch, F. Barre-Sinoussi, S.F.J. LeGrice and J.L. Darlix; HIV-1 Reverse Transcriptase Specifically Interacts with the Anticodon Domain of its Cognate Primer t-RNA; The EMBO Journal vol. 8, No. 11, pp. 3279-3285 (1989).
Wakefield et al. "Minimal Sequence Requirements of a Functional Human Immunodeficiency Virus Type 1 Primer Binding Sequence." J. of Virology vol. 68(3):1605-1614, 1994.
Atze et al. "Reduced Replication of Human Immunodeficiency Virus Type 1 Mutants That Use Reverse Transcriptase Primers other than the Natural tRNA.sub.3.sup.Lys " J. of Virology vol. 69(5):3090-3097, 1995.
Weiss et al. "Synthetic Human tRNA.sub.UUU.sup.Lys3 and natural bovine tRNA.sub.UUU.sup.Lys3 interact with HIV-1 reverse transcriptase and serve as specific primers for retroviral cDNA synthesis" Gene vol. 111:183-197, 1992.
Lund et al. "Mutated Primer Binding Sites Interating with Diffrent tRNAs Allow Efficient Murine Leukemia Virus Replication" J. of Virology vol. 67(12):7125-7130, 1993.
Krieg et al. "The use of a synthetic tRNA gene as a novel approach to study in vivo transcription and chromatin structure in yeast" NAR vol. 19(14):3849-3855, 1991.
Barat et al. "Interaction of HIV-1 reverse transcriptase with a synthetic form of its replication primer, tRNA.sup.Lys3 " NAR vol. 19(4):751-757, 1991.

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