Retroviral vector, a replication system for said vector and avia

Details Retroviral vector, a replication system for said vector and avia Retroviral vector, a replication system for said vector and avia

1998-10-09

2000-03-14

Elliott, George C.

Chemistry: molecular biology and microbiology

Vector, per se

435 691, 435440, 435455, 435325, 435349, 536 231, 536 2433, C07H 2104, C12N 1511, C12N 1563

Patent

active

060371722

ABSTRACT:
A retroviral vector comprising a retrovirus with infectivity for birds and/or mammals in which at least part of the genomic RNA sequences carrying information for the production of viral proteins required in trans for retroviral replication have been replaced by one or more sequences carrying information to be introduced in a target cell chromosome, characterized in that the primer binding site (PBS) has been modified to a sequence that does not allow strong base pairing with the 3' end or other priming sequence in any naturally occurring tRNA.

REFERENCES:
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Atze et al "Reduced Replications of Human Immunodeficiency Virus Type 1 Mutants that use Reverse Transcriptase Primers other than the Natural tRNA.sup.LYS 3 " J. of Virology vol. 69(5): 3090-3097, 1995.
Weiss et al "Synthetic Human tRNA.sup.LYS 3 uhe and Natural Bovine tRNA.sup.LYS 3 yU Interact with HIV-1 Reverse Transcriptase and Serve as Primers for Retroviral cDNA Synthesis" Gene vol. 111:83-197, 1992.
Lund et al "Murato Primer Binder Sites Interacting with Different tRNAS Allow Efficient Murine Leukemia Virus Replication" J of Virology vol. 67(12): 7125-7130, 1993.
Krieg et al "The Use of Synthetic tRNA Gene as a novel Approach to study in vivo Transcription and Chem. in Structure in Yeast" NAR vol. 19(14):3849-3855,1991.
Barat et al "Interaction of HIV-1 Reverse Transcriptase with a Synthetic Form of its Replication Primers, tRNA.sup.Lys 3 " NAR vol 19(4): 751-757, 1991.
Barat, C., V. Lullien, O. Schatz, G. Keith, M.T. Nugeyre, F. Gruninger-Leitch, F. Barre-Sinoussi, S.F.J. LeGrice and J.L. Darlix; HIV-1 Reverse Transcriptase Specifically Interacts with the Anticodon Domain of its Cognate Primer t-RNA; The EMBO Journal vol.8, No. 11, pp. 3279-3285 (1989).
Pedersen, F.S. et al., "Control Points for Retroviral Vector Safety: A Look at t MLV Replication Cycle", abstract of paper presented at Goslar, Germany, conferenc Sep. 18, 1994.
Lund et al., Journal of Virology, Feb. 1997, vol. 71, pp. 1191-1195.
Sang-Moo Kang et al., Journal of Virology, Jan. 1997, vol. 71, No. 1, pp. 207-217.
Isel et al., Journal of Biological Chemistry, Dec. 1993, vol. 288,, No. 34, pp. 25269-25272.
Wakefield, et al., Journal of Virology, Oct. 1995, vol. 69, No. 10, pp. 6021-6029.
Isel et al., J. Mol. Biol. (1995) 247, pp. 236-250.

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