Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1994-11-08
1997-12-02
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 691, 4353201, 536 241, C12N 1500, C12N 1509, C12N 1563, C12P 2106
Patent
active
056935080
ABSTRACT:
Novel retroviral vectors were constructed by making modifications to the Moloney murine leukemia virus (MoMLV) long terminal repeat (LTR). A portion of the U3 region of the MoMLV LTR was replaced with a hybrid regulatory element consisting of the human cytomegalovirus immediate-early enhancer/promoter (CMV-IE) together with the human immunodeficiency virus transactivation response element (HIV-TAR). Transfection of chloramphenicol acetyl transferase (CAT) reporter constructs into a variety of human cell lines showed that the CMV-IE/HIV-TAR enhancer/promoter chimeric MoMLV LTR exhibited basal expression levels which were 10- to 50-fold higher than those obtained from the wild-type MoMLV LTR enhancer/promoter. Expression from the recombinant LTR was further increased in the presence of the HIV-1 Tat protein. When stably transfected into an amphotropic packaging cell line, the modified retroviral vector containing the chimeric LTR plus an extended packaging signal consistantly gave higher titers of retrovirus than did the parental MoMLV based vector. These novel retroviral vectors provide improved means for the delivery and expression of genes in different cell types.
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Nucker Christine M.
Parkin Jeffrey S.
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