Reticulocyte analyzing method and apparatus utilizing light scat

Details Reticulocyte analyzing method and apparatus utilizing light scat Reticulocyte analyzing method and apparatus utilizing light scat

1995-11-17

1997-04-01

Warden, Jill

Chemistry: analytical and immunological testing

Biological cellular material tested

436172, 436175, 422 8201, 422 8202, 422 8205, 422 8208, 422 8209, 356 39, G01N 3348

Patent

active

056165013

ABSTRACT:
A reticulocyte analyzing method and apparatus in which a biological sample stream of ghosted red cells is passed into and through a point focused beam of light, such as laser light. A light detector structure is positioned with respect to the axis of the light beam to provide a light output pulse indicative of the passage of each cell. Electrically conductive contacts within the fluid stream can provide additional electrical pulse outputs of each cell. A staining reagent can be utilized with a ghosting reagent to further differentiate the reticulocytes. The light and the light and electronic produced output pulses or signals can be combined to define and quantify reticulocytes as distinct from other known cell types.

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