Restrictocin-like ribotoxin analogues comprising only one cystei

Details Restrictocin-like ribotoxin analogues comprising only one cystei Restrictocin-like ribotoxin analogues comprising only one cystei

1992-07-13

1995-08-08

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 691, 4351723, 4353201, 4352523, 4352401, 43525411, 536 232, 536 234, 935 10, 935 14, C12N 922, C12N 1555, C12N 121, C12N 510, C12N 115

Patent

active

054398150

ABSTRACT:
Ribotoxins, such as restrictocin, mitogillin and alpha-sarcin which do not contain a cysteine for linkage to antibodies for immunotoxin production need to be modified for such linkage without compromising biological activity. Protein analogues of such native ribotoxins are provided in which both the analogue and the native ribotoxin can cleave only a single phosphodiester bond of 28S rRNA in a 60S ribosomal subunit and in which the analogue comprises only one cysteine available from covalent linkage to a partner, such as an antibody, which cysteine is not present in the native ribotoxin. Especially preferred analogues are restrictocin Cys 13, Cys 82, Cys 106 and Cys 110 and restrictocin Cys 150-Gly151. Corresponding DNA sequences, vectors, host cells, immunotoxins and pharmaceutical compositions are also claimed.

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