Restriction/ligation labeling for primer initiated multiple copy

Details Restriction/ligation labeling for primer initiated multiple copy Restriction/ligation labeling for primer initiated multiple copy

1990-10-22

1994-11-22

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, C12N 1510, C12Q 168

Patent

active

053668774

ABSTRACT:
Sample DNA is analyzed by joining dsDNA sample fragments to labeling moieties having a primer binding sequence, to provide labeled dsDNA. After denaturation of the labeled dsDNA, strands binding to a probe are separated, conveniently using particles and a specific binding pair, followed by amplification of the sample strands and analysis and/or isolation of the amplified strands.

REFERENCES:
patent: 4672040 (1987-06-01), Josephson
patent: 4920061 (1990-04-01), Poynton et al.
patent: 5093245 (1992-03-01), Keith et al.
Kempe et al. (1985), Nucl. Acids Res. 13(1): 45-57.

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