Restriction-fragment DNA probes and probe clusters

Details Restriction-fragment DNA probes and probe clusters Restriction-fragment DNA probes and probe clusters

1990-06-15

1992-06-02

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 91, 536 27, C12Q 168, C12P 1934, C07H 1512

Patent

active

051186047

ABSTRACT:
A probe capable of binding by homologous base pairing independently to a pair of gene regions bordering the upstream and downstream sides of a pair of infrequent restriction endonuclease sites separated by between about 20-2,000 kilibases on a section of linear DNA. The probe is produced, according to the method of the invention by digesting the DNA section to completion with the selected endonuclease, and ligating the resulting fragments under conditions which favor end-to-end circularization, and selecting digest fragments of the large circular molecules which have end to end junctions. Also disclosed are method for mapping and ordering the positions of such probes, using another set of linking probes which span such rate cutting sites.

REFERENCES:
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Steinmetz, M. et al., Nature 300:35 (1982).
Rackwitz, H-R. et al. Gene 30:195 (1984).
Seed, B., Nucl. Acids Res. 11 (8):2427 (1983).
Serwer, P., Biochem. 19:3001 (1980).
Gusella, J. F. et al., Naute 306:234 (1983).
Dunn, R. J. et al., J. Biol. Chem. 256(12):6109 (1981).
Potter, H. et al., Proc. Natl. Acad. Sci. USA 76(3):1084 (1979).
Lau, Y-F. et al., Proc. Natl. Acad. Sci. USA 80:5225 (1983).

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