Restriction endonuclease obtainable foam bacillus coagulans and

Details Restriction endonuclease obtainable foam bacillus coagulans and Restriction endonuclease obtainable foam bacillus coagulans and

1990-07-02

1993-04-06

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435832, C12N 922, C12N 100

Patent

active

052003361

ABSTRACT:
The present invention provides a novel Type II restriction endonuclease obtainable from Bacillus coagulans. The endonuclease known as Bcg I, recognizes the following nucleotide sequence and has a cleavage point at both ends outside of its recognition sequence: ##STR1## to produce a 34 base pair fragment. Also described is a process for obtaining purified Bcg I from Bacillus coagulans, as well as processes for mapping chromosomal DNA and methods for reducing background in transformants with enzymes such as Bcg I.

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